Detailed Information on Publication Record
2007
Involvement of intracellular chloride and promiscuous channels in apoptosis and cardioprotection
ONDRIAŠ, Karol, Lubica MALEKOVÁ, Vladimíra KOMÍNKOVÁ, Juraj KOPÁČEK, Olga KRIŽANOVÁ et. al.Basic information
Original name
Involvement of intracellular chloride and promiscuous channels in apoptosis and cardioprotection
Name in Czech
Úloha nitrobuněčných chloridových a nespecifických kanálů v apoptóze a kardioprotekci
Authors
ONDRIAŠ, Karol, Lubica MALEKOVÁ, Vladimíra KOMÍNKOVÁ, Juraj KOPÁČEK, Olga KRIŽANOVÁ and Marie NOVÁKOVÁ
Edition
Acta Physiologica, Oxford, Blackwell Publishing, 2007, 1748-1708
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30105 Physiology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.602
Organization unit
Faculty of Medicine
Keywords in English
chloride channels;mitochondrial promiscuos channels;apoptosis;cardioprotection
Tags
International impact, Reviewed
Změněno: 19/6/2009 15:54, prof. MUDr. Marie Nováková, Ph.D.
V originále
Aims: Purpose of the study was to test the hypothesis that intracellular chloride and mitochondrial promiscuous channels are involved in the apoptosis and cardioprotection. Methods: We measured effects of the chloride blockers DIDS, NPPB and Phloretin on H2O2-induced cardiomyocyte apoptosis and single channel properties of chloride and high conductance promiscuous channels derived from rat heart lysosomal and mitochondrial membranes incorporated in bilayer lipid membrane (BLM). Promiscuity of the single mitochondrial channels was measured as a voltage change of a voltage-dependent switch from K+ to Cl- selectivity and an opposite switch. Results: The chloride channel blockers (100 micromol/l) inhibited the H2O2 induced cardiomyocytes apoptosis. They inhibited the chloride channels at concentrations of 10-50 micromol/l by decreasing the channel open probability and the open dwell time. They affected promiscuity of the mitochondrial high conductance channels, influencing the voltage-switch and the K+/Cl- channel activity. Conclusion: The obtained results did not contradict the tested hypothesis. We may assume that the intracellular and the promiscuous channels may be involved in apoptosis and cardioprotection, however a definite proof is still missing. The results may contribute to understand a possible involvement of intracellular chloride and promiscuous channels in apoptosis and cardioprotection.
In Czech
Aims: Purpose of the study was to test the hypothesis that intracellular chloride and mitochondrial promiscuous channels are involved in the apoptosis and cardioprotection. Methods: We measured effects of the chloride blockers DIDS, NPPB and Phloretin on H2O2-induced cardiomyocyte apoptosis and single channel properties of chloride and high conductance promiscuous channels derived from rat heart lysosomal and mitochondrial membranes incorporated in bilayer lipid membrane (BLM). Promiscuity of the single mitochondrial channels was measured as a voltage change of a voltage-dependent switch from K+ to Cl- selectivity and an opposite switch. Results: The chloride channel blockers (100 micromol/l) inhibited the H2O2 induced cardiomyocytes apoptosis. They inhibited the chloride channels at concentrations of 10-50 micromol/l by decreasing the channel open probability and the open dwell time. They affected promiscuity of the mitochondrial high conductance channels, influencing the voltage-switch and the K+/Cl- channel activity. Conclusion: The obtained results did not contradict the tested hypothesis. We may assume that the intracellular and the promiscuous channels may be involved in apoptosis and cardioprotection, however a definite proof is still missing. The results may contribute to understand a possible involvement of intracellular chloride and promiscuous channels in apoptosis and cardioprotection.