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@proceedings{725670, author = {Dubový, Petr and Klusáková, Ilona and Svíženská, Ivana and Jančálek, Radim}, booktitle = {European Glial Cell Meeting}, keywords = {satellite cells; chemokine; chemokine receptor}, language = {eng}, isbn = {1740-925X}, title = {An immunohistochemical localization of SDF-1 and its receptor CXCR4 in dorsal root ganglia associated and non-associated to damaged nerve}, url = {http://journals.cambridge.org/action/displayJournal?jid=NGB}, year = {2007} }
TY - CONF ID - 725670 AU - Dubový, Petr - Klusáková, Ilona - Svíženská, Ivana - Jančálek, Radim PY - 2007 TI - An immunohistochemical localization of SDF-1 and its receptor CXCR4 in dorsal root ganglia associated and non-associated to damaged nerve SN - 1740925X KW - satellite cells KW - chemokine KW - chemokine receptor UR - http://journals.cambridge.org/action/displayJournal?jid=NGB N2 - The biological activity of stromal cell-derived factor (SDF1) is signaled through the chemokine receptor CXCR4. An immunohistochemical location of SDF-1 and CXCR4 proteins was investigated in the C7-C8 and L4-L5 dorsal root ganglia (DRG) of naive rats and those operated for unilateral L4-L5 spinal nerve ligature (SNL) and sciatic nerve ligature (ScNL). The naive DRG displayed immunofluorescence for SDF1-IF and CXCR4-IF at the surface of neurons and satellite glial cells (SGC). A significant decrease of SDF1-IF was induced in the ipsilateral, but remained distinct in the contralateral L4-L5 DRG one week after SNL. A bilateral reduction of SDF1-IF was found 2 weeks from SNL. A gradual diminution of SDF1-IF was observed bilaterally in the L4-L5 DRG neurons, but modest IF was in SGC after 1 and 2 weeks from ScNL, respectively. The SNL for 1 week induced a reduction of CXCR4-IF at the SGC surfaces of ipsi- in contrast to contralateral L4-L5 DRG, while intraneuronal staining was increased. Two weeks after SNL, the differences of CXCR4-IF at the neuronal surfaces in ipsilateral L4-L5 DRG and at the SGC surfaces in contralateral ones were marked. CXCR4-IF at the SGC surfaces remained in both ipsi- and contralateral DRG up to 2 weeks from ScNL, but it was increased intraneuronally only in the ipsilateral DRG. Similar pattern of SDF1- and CXCR4-IF was induced in the cervical DRG. A different expression of SDF1-IF and its receptor was observed in ipsi- and contralateral DRG associated and non-associated with unilateral SNL and ScNL. This suggests their different regulation with the time of survival and type of nerve injury. ER -
DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana SVÍŽENSKÁ and Radim JANČÁLEK. An immunohistochemical localization of SDF-1 and its receptor CXCR4 in dorsal root ganglia associated and non-associated to damaged nerve. In \textit{European Glial Cell Meeting}. 2007. ISBN~1740-925X.
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