A simple procedure for isolation of PCR-ready bacterial DNA
| ŠPANOVÁ, Alena, Bohuslav RITTICH, Jana KŘÍŽOVÁ, Eva NĚMCOVÁ and Lucie KORDAČOVÁ. A simple procedure for isolation of PCR-ready bacterial DNA. Online. In Ninth International Symposium on Advances in Extraction Technologies. Aalesund, Norway: University of Oslo, 2007. p. 209-209. [citováno 2024-04-23] |
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| Basic information | |
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| Original name | A simple procedure for isolation of PCR-ready bacterial DNA |
| Name in Czech | Jednoduchý postup izolace bakteriální DNA v kvalitě vhodné pro PCR |
| Authors | ŠPANOVÁ, Alena (203 Czech Republic), Bohuslav RITTICH (203 Czech Republic, guarantor), Jana KŘÍŽOVÁ (203 Czech Republic), Eva NĚMCOVÁ (203 Czech Republic) and Lucie KORDAČOVÁ (203 Czech Republic) |
| Edition | Aalesund, Norway, Ninth International Symposium on Advances in Extraction Technologies, p. 209-209, 1 pp. 2007. |
| Publisher | University of Oslo |
| Other information | |
|---|---|
| Original language | English |
| Type of outcome | Proceedings paper |
| Field of Study | Genetics and molecular biology |
| Country of publisher | Norway |
| Confidentiality degree | is not subject to a state or trade secret |
| RIV identification code | RIV/00216224:14310/07:00022639 |
| Organization unit | Faculty of Science |
| Keywords in English | DNA; isolation; salting-out; NaCl |
| Tags | DNA, isolation, NaCl, salting-out |
| Tags | International impact |
| Changed by | Changed by: doc. Ing. Bohuslav Rittich, CSc., učo 1639. Changed: 16/7/2008 12:44. |
| Abstract |
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| PCR-ready DNA was isolated using salting-out procedure from crud cell lysates. The evaluated method is suitable for DNA isolation from pure cultures of bacterial cells. The reproducibility of RAPD and rep-PCR paterns was comparable with phenol extraction procedure. |
| Abstract (in Czech) |
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| Cílem práce byla izoloce DNA v kvalitě pro PCR z hrubých lyzátů buněk bakterií pomocí vysolování chloridem sodným.Navržená metoda je vhodná pro izolaci DNA z čistých bakteriálních kultur. Reprodukovatelnost RAPD a rep-PCR profilů je srovnatelná s fenolovou extrakcí. |
| Links | |
|---|---|
| MSM0021622415, plan (intention) | Name: Molekulární podstata buněčných a tkáňových regulací |
| Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations | |
| 1G46045, research and development project | Name: Molekulárně biologická analýza tuzemského fondu produkčních kmenů baktérií mléčného kvašení. |
| Investor: Ministry of Agriculture of the CR, Introduction of new traits for quality and stability improving in variety production of selcted leguminous species by hybridization methods | |
| 1G58097, research and development project | Name: Molekulárně-genetické metody pro identifikaci probiotických bakterií lidského původu s vhodnými technologickými a hygienicko-zdravotními vlastnostmi pro výrobu funkčních potravin. |
| Investor: Ministry of Agriculture of the CR, Molecular-genetic methods for identification of probiotic bakteria with good technologic, hygienic, and health properties | |
| 2B06053, research and development project | Name: Nové postupy při charakterizaci a identifikaci probiotických kmenů bakterií vhodných pro funkční potraviny. |
| Investor: Ministry of Education, Youth and Sports of the CR, New methods for the characterization and identification of probiotic bacterial strains suitable for functional foods | |
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