Detailed Information on Publication Record
2007
Molecular characteristics of Bifidobacterium strains collected in the Czech Collection of Microorganisms (CCM) and Laktoflora(CCDM) and reclassification of Bifidobacterium adolescentis CCM 3761 as Bifidobacterium pseudocatenulatum
RITTICH, Bohuslav, Alena ŠPANOVÁ, Radka BURDYCHOVÁ, Jana KŘÍŽOVÁ, Vladimír DRÁB et. al.Basic information
Original name
Molecular characteristics of Bifidobacterium strains collected in the Czech Collection of Microorganisms (CCM) and Laktoflora(CCDM) and reclassification of Bifidobacterium adolescentis CCM 3761 as Bifidobacterium pseudocatenulatum
Name in Czech
Molekulární charakterizace kmenů Bifidobacterium uložených ve České sbírce mikroorganismů (CCM) a Laktoflora (CCDM) a reklasifikace Bifidobacterium adolescentis CCM 3761 na Bifidobacterium pseudocatenulatum
Authors
RITTICH, Bohuslav (203 Czech Republic, guarantor), Alena ŠPANOVÁ (203 Czech Republic), Radka BURDYCHOVÁ (203 Czech Republic), Jana KŘÍŽOVÁ (203 Czech Republic), Vladimír DRÁB (203 Czech Republic) and Jan DRBOHLAV (203 Czech Republic)
Edition
Aas, Norway, 2nd Symposium on Propionibacteria and Bifidobacteria, p. 13P, 1 pp. 2007
Publisher
Norwegian University of Life Sciences, Aas, Norway
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
Genetics and molecular biology
Country of publisher
Norway
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/07:00022646
Organization unit
Faculty of Science
Keywords in English
Bifidobacterium; identification; PCR; ARDRA; rep-PCR
Tags
Tags
International impact
Změněno: 27/12/2007 19:07, doc. Ing. Bohuslav Rittich, CSc.
V originále
Sixteen Bifidobacterium strains of eleven species were used to evaluate the discrimination power of the tested methods. The strains were obtained from the Czech Collection of Microorganisms (CCM, Brno, Czech Republic) and Laktoflora (CCDM, Culture Collection of Dairy Microorganisms (Tábor, Czech Republic). DNA was amplified using two different genus-specific Bifidobacterium primer sets targeted on the 16S rDNA region. Amplicons 914 bp and 523 bp were used for the restriction analysis using BamHI, Sau3AI, TaqI, Sau96I, AluI, and NciI endonucleases. Seven random primers and BOXA1R and (GTG)5 primers were used for the evaluation of RAPD and rep-PCR, respectively. When characterizing the studied strains it was found that the results obtained for the strain B. adolescentis CCM 3761 did not correspond with its taxonomic position and the strain should be reclassified as B. pseudocatenulatum. The amplicons of the 16S rDNA region of B. adolescentis CCM 3761 and B. catenulatum DSM 16992T, B. pseudocatenulatum CCM 7094T, and B. adolescentis CCM 4987T were used for sequencing and for confirmation of species identification.
In Czech
Šestnáct kmenů jedenácti druhů bakterií Bifidobacterium bylo použito k ověření testovaných metod. Kmeny byly získány České sbírky mikroorganismů (CCM, Brno) a Laktoflora (CCDM, Tábor). DNA byla amplifikována pomocí dvou rozdílných rodově specifických primerů z oblasti 16S rDNA. Pro restrikční analýzu byly použity amplikony dlouhé 914 a 523 bp. Byly pouzity restriktasy BamHI, Sau3AI, TaqI, Sau96I, AluI a NciI. Pro RAPD a rep-PCR bylo použito sedm náhodných primerů, případně primery BOXA1R a (GTG)5. bylo zjištěno, že zařazení kmene B. adolescentis CCM 3761 neodpovídá jeho taxonomické pozici a kmen byl reklasifikován jako B. pseudocatenulatum. Pro potvrzení identifikace kmenů byly sekvenovány amplikony oblasti 16S rDNA u kmenů B. adolescentis CCM 3761, B. catenulatum DSM 16992T, B. pseudocatenulatum CCM 7094T a B. adolescentis CCM 4987T.
Links
| MSM0021622415, plan (intention) |
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| QF3169, research and development project |
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