J 2007

Experimental design in intraspecific organelle DNA sequence studies III: statistical measures of sampling success

HOLT, Jason A, Sierra Dawn STONEBERG HOLT and Petr BUREŠ

Basic information

Original name

Experimental design in intraspecific organelle DNA sequence studies III: statistical measures of sampling success

Name in Czech

Experimental design in intraspecific organelle DNA sequence studies III: statistical measures of sampling success

Authors

HOLT, Jason A (840 United States of America), Sierra Dawn STONEBERG HOLT (840 United States of America) and Petr BUREŠ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Taxon, Vienna, International Assoc. for Plant Taxonomy, 2007, 0040-0262

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.524

RIV identification code

RIV/00216224:14310/07:00022667

Organization unit

Faculty of Science

UT WoS

000249247400020

Keywords in English

bulking; haplotype frequency confidence intervals; inclusion-exclusion principle; intraspecific sequence studies; phylogeography; proportion estimates

Tags

International impact, Reviewed
Změněno: 21/4/2011 12:47, prof. RNDr. Petr Bureš, Ph.D.

Abstract

V originále

Statistical methods are proposed for analyzing the experimental design, preliminary results, and final results of phylogenetic studies of organelle DNA sequence at low taxonomic levels. Such studies require sampling numerous individuals, many of which share identical haplotypes. The proportions of the haplotypes sampled can help answer the following questions: (1) Is one haplotype so dominant that the particular DNA region is without meaningful variation within the scope of the study? (2) Were all prevalent haplotypes found? (3) What are the proportions of each haplotype within the studied group? (4) What percentage of the studied group can be confidently asserted to belong to the haplotypes that were found? Examples are given in which the statistics techniques are applied to data drawn from the botanical literature. Tables are included as a quick reference for the researcher who wishes to circumvent calculation. A Microsoft Excel 2000 spreadsheet (titled HaploPro.xls) for performing some of the more complicated calculations is offered online. Finally, the limitations of these methods and their applicability to nuclear DNA and other characters studies are discussed.

In Czech

Statistical methods are proposed for analyzing the experimental design, preliminary results, and final results of phylogenetic studies of organelle DNA sequence at low taxonomic levels. Such studies require sampling numerous individuals, many of which share identical haplotypes. The proportions of the haplotypes sampled can help answer the following questions: (1) Is one haplotype so dominant that the particular DNA region is without meaningful variation within the scope of the study? (2) Were all prevalent haplotypes found? (3) What are the proportions of each haplotype within the studied group? (4) What percentage of the studied group can be confidently asserted to belong to the haplotypes that were found? Examples are given in which the statistics techniques are applied to data drawn from the botanical literature. Tables are included as a quick reference for the researcher who wishes to circumvent calculation. A Microsoft Excel 2000 spreadsheet (titled HaploPro.xls) for performing some of the more complicated calculations is offered online. Finally, the limitations of these methods and their applicability to nuclear DNA and other characters studies are discussed.

Links

LC06073, research and development project
Name: Centrum pro výzkum biodiverzity
Investor: Ministry of Education, Youth and Sports of the CR, Biodiversity Research Center
MSM0021622416, plan (intention)
Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time