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@inproceedings{726038,
author = {Španová, Alena and Rittich, Bohuslav and Horák, Daniel and Rybnikář, Alois},
address = {Budapest, Hungary},
booktitle = {Biopowders; Advances in the Powder Technology},
keywords = {fungi; Trichophyton; nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA)},
language = {eng},
location = {Budapest, Hungary},
isbn = {978-963-9696-23-5},
pages = {159-164},
publisher = {Research Institute of Chemical and Process Enginnering, University of Pannonia, Vesazprém, Hungary},
title = {Isolation of PCR-ready DNA from real samples containing PCR inhibitors},
year = {2007}
}
TY - JOUR
ID - 726038
AU - Španová, Alena - Rittich, Bohuslav - Horák, Daniel - Rybnikář, Alois
PY - 2007
TI - Isolation of PCR-ready DNA from real samples containing PCR inhibitors
PB - Research Institute of Chemical and Process Enginnering, University of Pannonia, Vesazprém, Hungary
CY - Budapest, Hungary
SN - 9789639696235
KW - fungi
KW - Trichophyton
KW - nonporous magnetic hydrophilic microspheres
KW - P(HEMA-co-EDMA)
KW - P(HEMA-co-GMA)
N2 - Hydrophilic magnetic particles coated by carboxyl groups were used for PCR-ready DNA isolation. DNA was adsorbed on magnetic particles in the presence of 8 wt. % of poly(ethylene glycol) (PEG 6,000) and 2 M NaCl concentrations, washed and eluted into TE buffer (10 mM Tris-HCl, pH 7.8; 1 mM EDTA; pH 8.0). The eluted DNA was used directly for PCR amplification and identification of Lactobacillus and Bifidobacterium cells in dairy products (cheese, fermented dairy products) and lyophilisates, Trichophyton cells in mixed cultures and bacterial cells in sediments from Lake Baikal. It was also used in GMO testing in foods with success.
ER -
ŠPANOVÁ, Alena, Bohuslav RITTICH, Daniel HORÁK and Alois RYBNIKÁŘ. Isolation of PCR-ready DNA from real samples containing PCR inhibitors. In \textit{Biopowders; Advances in the Powder Technology}. Budapest, Hungary: Research Institute of Chemical and Process Enginnering, University of Pannonia, Vesazprém, Hungary, 2007, p.~159-164. ISBN~978-963-9696-23-5.
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