A 2007

Coupling of microcolumn separations to MALDI MS

PREISLER, Jan

Basic information

Original name

Coupling of microcolumn separations to MALDI MS

Name in Czech

Spojení mikrokolonových separací s MALDI MS

Authors

PREISLER, Jan (203 Czech Republic, guarantor, belonging to the institution)

Edition

Vienna, Mass Spec Forum, 2007

Publisher

University of Vienna

Other information

Language

English

Type of outcome

Audiovizuální tvorba

Field of Study

10406 Analytical chemistry

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

RIV identification code

RIV/00216224:14310/07:00024581

Organization unit

Faculty of Science

Keywords in English

MALDI; MS; ESI; deposition; interface; CE
Změněno: 9/4/2013 14:11, prof. Mgr. Jan Preisler, Ph.D.

Abstract

V originále

Electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) are preferred ionization techniques in analysis of polymers. While ESI is usually chosen for on-line coupling of column separations to MS, MALDI is used for MS screening of large number of samples. Certain features of MALDI, however, make it more attractive in some applications: MALDI spectra, dominated by singly charged ions, are simpler and solid samples prepared on MALDI targets can be archived. This lecture will give an overview of approaches for coupling of microLC and CE with MALDI MS. The power of separation coupled a TOF/TOF instrument will be demonstrated on sensitive multidimensional analysis of complex biological samples. In conjunction with TOF mass spectrometer and high repetition rate lasers, extremely fast analyses can be achieved. The potential for high throughput MS analysis will be discussed on example of on-line and off-line analysis.

In Czech

Souhrnná přednáška o spojení mikrokolonových separačních technik s MALDI TOF MS

Links

GA203/03/0515, research and development project
Name: Integrovaná analýza genomu a proteomu terapeuticky významných bakteriofágů kombinací elektroforézy a hmotnostní spektrometrie
Investor: Czech Science Foundation, Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry
LC06035, research and development project
Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.
Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations