Degradation of Beta-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Hexachlorocyclohexane-Utilizing Bacterium Sphingobium sp. MI1205

ITO, M., Zbyněk PROKOP, Martin KLVAŇA, Y. OTSUBO, M. TSUDA, Jiří DAMBORSKÝ and Yuji NAGATA. Degradation of Beta-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Hexachlorocyclohexane-Utilizing Bacterium Sphingobium sp. MI1205. ARCHIVES IN MICROBIOLOGY. 2007, vol. 188, No 4, p. 313-325. ISSN 0302-8933.

Basic information

• Original name: Degradation of Beta-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Hexachlorocyclohexane-Utilizing Bacterium Sphingobium sp. MI1205
• Name in Czech: Degradace Beta-Hexachlorocyclohexanu pomocí haloalkan dehalogenázy LinB získané bakterie využívajícící Hexachlorocyclohexan- Bacterium Sphingobium sp. MI1205
• Authors: ITO, M. (392 Japan), Zbyněk PROKOP (203 Czech Republic), Martin KLVAŇA (203 Czech Republic), Y. OTSUBO (392 Japan), M. TSUDA (392 Japan), Jiří DAMBORSKÝ (203 Czech Republic, guarantor) and Yuji NAGATA (392 Japan).
• Edition: ARCHIVES IN MICROBIOLOGY, 2007, 0302-8933.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 1.838
• RIV identification code: RIV/00216224:14310/07:00022810
• Organization unit: Faculty of Science
• UT WoS: 000249522600003
• Keywords in English: hexachlorocyclohexane (HCH); Sphingobium japonicum UT26; Sphingobium sp. MI1205; haloalkane dehalogenase LinB
• Tags: haloalkane dehalogenase LinB, hexachlorocyclohexane (HCH), Sphingobium japonicum UT26, Sphingobium sp. MI1205
• Tags: International impact, Reviewed

Abstract

The technical formulation of hexachlorocyclohexane (HCH) mainly consists of the insecticidal gamma-isomer and noninsecticidal alpha, beta, and delta-isomers, among which beta-HCH is the most recalcitrant and has caused serious environmental problems. A gamma-HCH-utilizing bacterial strain, Sphingobium sp. MI1205, was isolated from soil which had been contaminated with HCH isomers. This strain degraded beta-HCH more rapidly than the well-characterized gamma-HCH-utilizing strain Sphingobium japonicum UT26. In MI1205, beta-HCH was converted to 2,3,5,6-tetrachlorocyclohexane-1,4-diol (TCDL) via 2,3,4,5,6-pentachlorocyclohexanol (PCHL). A haloalkane dehalogenase LinB (LinB-MI) that is 98 % identical (seven amino-acid differences among 296 amino acids) to LinB from UT26 (LinB-UT) was identified as an enzyme responsible for the two-step conversion of beta-HCH to TCDL. This property of LinB-MI contrasted with that of LinB-UT, which catalyzed only the first step conversion of beta-HCH to PCHL. Site-directed mutagenesis and computer modeling suggested that two of the seven different amino acid residues (V134 and H247) forming a catalytic pocket of LinB are important for the binding of PCHL in an orientation suitable for the reaction in LinB-MI. However, mutagenesis also indicated the involvement of other residues for the activity unique to LinB-MI. Sequence analysis revealed that MI1205 possesses the IS6100-flanked cluster that contains two copies of the linB-MI gene. This cluster is identical to the one located on the exogenously isolated plasmid pLB1, suggesting that MI1205 had recruited the linB genes by a horizontal transfer event.

Abstract (in Czech)

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Links

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)