Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110.

SATO, Yukari, Rio NATSUME, M. TSUDA, Jiří DAMBORSKÝ, Yuji NAGATA and T. SENDA. Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110. Acta Crystallographica Section F. 2007, vol. 63, No 4, p. 294-296. ISSN 1744-3091.

Basic information

• Original name: Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110.
• Name in Czech: Krystalizace a zakladní krystalografická analýza haloalkan dehalogenázy DbjA z bakterie Bradyrhizobium japonicum USDA110.
• Authors: SATO, Yukari (392 Japan), Rio NATSUME (392 Japan), M. TSUDA (392 Japan), Jiří DAMBORSKÝ (203 Czech Republic, guarantor), Yuji NAGATA (392 Japan) and T. SENDA (392 Japan).
• Edition: Acta Crystallographica Section F, 2007, 1744-3091.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 0.645
• RIV identification code: RIV/00216224:14310/07:00022814
• Organization unit: Faculty of Science
• UT WoS: 000245505800010
• Keywords in English: Haloalkane dehalogenases; degradation of halogenated aliphatic pollutants; DbjA; crystals
• Tags: crystals, DbjA, Haloalkane dehalogenases
• Tags: International impact, Reviewed

Abstract

Haloalkane dehalogenases are key enzymes for the degradation of halogenated aliphatic pollutants. A haloalkane dehalogenase, DbjA, constitutes a novel substrate specificity class with high catalytic activity for beta-methylated haloalkanes. In order to reveal the mechanism of the substrate specificity, DbjA has been crystallized by the hanging drop vapor diffusion method. The best crystals were obtained by the microseeding technique with a reservoir solution of 17-19.5% (w/v) PEG 4000, 0.2 M calcium acetate, and 0.1 M Tris-HCl (pH 7.7-8.0). The space group of the DbjA crystal is P212?2 with unit cell parameters a=212.85 A, b=117.84 A, c=55.80 A. The crystal diffracts to 1.75 A resolution.

Abstract (in Czech)

xxx

Links

• LC06010, research and development project: Name: Centrum biokatalýzy a biotransformací

Investor: Ministry of Education, Youth and Sports of the CR, Center of Biocatalysis and Biotransformation