Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry

MONINCOVÁ, Marta, Zbyněk PROKOP, Jitka VEVODOVÁ, Yuji NAGATA and Jiří DAMBORSKÝ. Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry. Applied and Environmental Microbiology. 2007, vol. 73, No 6, p. 2005-2008. ISSN 0099-2240.

Basic information

• Original name: Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry
• Name in Czech: Slabá aktivita haloalkan dehalogenázy LinB s 1,2,3-Trichloropropanem srovnávání pomocí X-ray Krystalografie a Microkalorimetrie.
• Authors: MONINCOVÁ, Marta (203 Czech Republic), Zbyněk PROKOP (203 Czech Republic), Jitka VEVODOVÁ (203 Czech Republic), Yuji NAGATA (392 Japan) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor).
• Edition: Applied and Environmental Microbiology, 2007, 0099-2240.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 4.004
• RIV identification code: RIV/00216224:14310/07:00022815
• Organization unit: Faculty of Science
• UT WoS: 000245156800039
• Keywords in English: 1;2;3-trichloropropane (TCP); Haloalkane dehalogenases; LinB from Sphingobium japonicum UT26; X-ray crystallography; microcalorimetry
• Tags: 1, 2, 3-trichloropropane (TCP), Haloalkane dehalogenases, microcalorimetry, X-ray crystallography
• Tags: International impact, Reviewed

Abstract

,2,3-trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes catalysing the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP since the reaction can not be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (kcat = 0.005 s-1) of LinB with TCP using X-ray crystallography and microcalorimetry. This observation makes LinB a useful starting material for development of new biocatalyst towards TCP by protein engineering. Microcalorimetry is proposed to be a universal method for detection of weak enzymatic activities. Detection of these activities is becoming increasingly important for engineering of novel biocatalysts using the scaffolds of proteins with promiscuous activities.

Abstract (in Czech)

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Links

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)