Detailed Information on Publication Record
2007
Piezoelectric Immunosensor for the Direct and Rapid Detection of Francisella tularensis
POHANKA, Miroslav and Petr SKLÁDALBasic information
Original name
Piezoelectric Immunosensor for the Direct and Rapid Detection of Francisella tularensis
Name in Czech
Piezoelektrický imunosensor pro přímou a rychlou detekci Francisella tularensis
Authors
POHANKA, Miroslav (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor)
Edition
Folia Microbiologica, Prague, Czechoslovak Society for Microbiology, 2007, 0015-5632
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 0.989
RIV identification code
RIV/00216224:14310/07:00022880
Organization unit
Faculty of Science
UT WoS
000250290500002
Keywords in English
Piezoelectric sensor; Quartz crystal microbalance; Immunoassay
Tags
International impact, Reviewed
Změněno: 10/11/2007 14:22, prof. RNDr. Petr Skládal, CSc.
V originále
A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor included mouse polyclonal antibody immobilized through the layer of protein A covalently linked to the gold electrode of the sensor. Thus obtained immunosensor was able to detect F. tularensis with the limit of detection 105 CFU/mL with a typical measuring cycle under 5 min. The sensor was successfully evaluated for rapid detection of F. tularensis spikes in drinking water and milk; no deterioration of sensitivity in comparison with buffer solutions was observed. The proposed concept of a rapid measurement of microbial agents seems to be promising for evaluation of samples after short pre-cultivation enrichment.
In Czech
Byl vyvinut piezoelektrický imunosensor pro rychlé (pod 5 min) stanovení bakterie Francisella tularensis ve vodě. Imunosensor umožňuje několikanásobné stanovení bez regenerace citlivého povrchu.
Links
| LC06030, research and development project |
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