In vitro and in silico protein engineering of lectins

WIMMEROVÁ, Michaela. In vitro and in silico protein engineering of lectins. In 14th European Carbohydrate Symposium, EUROCARB 14. Lubeck (Germany): Research Center Borstel and the Institute of Chemistry, 2007, p. 147.

Basic information

Original name

In vitro and in silico protein engineering of lectins

Name in Czech

Proteinové inženýrství lektinů in vitro a in silico

Authors

WIMMEROVÁ, Michaela

Edition

Lubeck (Germany), 14th European Carbohydrate Symposium, EUROCARB 14, p. 147-147, 2007

Publisher

Research Center Borstel and the Institute of Chemistry

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Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Keywords in English

mutagenesis; structure; lectin; pathogen

Tags

lectin, mutagenesis, pathogen, structure

Tags

International impact

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Abstract

V originále

The contribution is focused on bacterial lectins from human opportunistic pathogens, which display sub-micromolar range affinity towards their carbohydrate ligands, targeting specially proteins from bacteria dangerous for cystic fibrosis patients. The combination of binding experiments (isothermal titration microcalorimetry, surface plasmon resonance,...) and X-ray crystallography approaches is used to decipher the thermodynamical and structural basis for high affinity binding of these lectins to host carbohydrates. Site-directed mutagenesis in combination with structural and functional studies is used to understandthe role of particular amino acids for sugar specificity and preference. In order to explore these interesting features deeper, we have been using a computational chemistry approach with the graphic program TRITON that is being under development at our institute. TRITON automates in silico engineering of proteins by generation of desired mutant proteins and performs docking experiments using AUTODOCK [2]. The TRITON results are correlated with experimental data.

In Czech

Proteinové inženýrství lektinů in vitro a in silico

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Links

LC06030, research and development project	Name: Biomolekulární centrum Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre
MSM0021622413, plan (intention)	Name: Proteiny v metabolismu a při interakci organismů s prostředím Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment