Laser diode (405 nm) as an excitation source in capillary electrophoresis with laser-induced fluorescence detection analysis of GFP-tagged proteins

RYVOLOVÁ, Markéta, Ondřej PEŠ, Radka FOHLEROVÁ, Jan HEJÁTKO and Jan PREISLER. Laser diode (405 nm) as an excitation source in capillary electrophoresis with laser-induced fluorescence detection analysis of GFP-tagged proteins. In CECE 2007 4th international interdisciplinary meeting on bioanalysis. první. Brno: Institute of Analytical Chemistry AS CR, 2007, p. 41-42, 1 pp. ISBN 978-80-254-0427-0.

Basic information

• Original name: Laser diode (405 nm) as an excitation source in capillary electrophoresis with laser-induced fluorescence detection analysis of GFP-tagged proteins
• Name in Czech: Laserová dioda (405 nm) jako excitační zdroj v kapilární elektroforéze s laserem indukovanou fluorescenční detkcí v analýze GFP značených proteinů
• Authors: RYVOLOVÁ, Markéta, Ondřej PEŠ, Radka FOHLEROVÁ, Jan HEJÁTKO and Jan PREISLER.
• Edition: první. Brno, CECE 2007 4th international interdisciplinary meeting on bioanalysis, p. 41-42, 1 pp. 2007.
• Publisher: Institute of Analytical Chemistry AS CR

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Science
• ISBN: 978-80-254-0427-0
• Keywords in English: capillary electrophoresis; GFP
• Tags: Capillary electrophoresis, GFP

Abstract

In this work, using laser diode (405 nm) as a prospective excitation source for monitoring of phosphorylation process of cytosolic histidine-containing phosphotransfer proteins (designated as AHP1-6 in Arabidopsis) has been suggested. To our knowledge, this laser emitting at 405 nm has not been applied for CE-LIF of GFP and/or GFP-tagged proteins yet.

Abstract (in Czech)

V této práci je navrženo použití laserové diody (405 nm) jako excitačního zdroje pro GFP značené proteiny, především pro monitorování fosforylačních procesů proteinů AHP

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations