Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms

NOVÁKOVÁ, Dana, Pavel ŠVEC and Ivo SEDLÁČEK. Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms. In Proceedings of the 11th International Conference on Culture Collections. 2007. ISBN 978-3-00-022417-1.

Basic information

• Original name: Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms
• Name in Czech: Charakterizace kmenů Staphylococcus sp. deponovaných v České sbírce mikroorganismů
• Authors: NOVÁKOVÁ, Dana (203 Czech Republic, guarantor), Pavel ŠVEC (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic).
• Edition: Proceedings of the 11th International Conference on Culture Collections, 2007.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/07:00023007
• Organization unit: Faculty of Science
• ISBN: 978-3-00-022417-1
• Keywords in English: Staphylococcus sp.; ribotyping; whole-cell protein analysis; rep-PCR
• Tags: rep-PCR, ribotyping, Staphylococcus sp., whole-cell protein analysis

Abstract

The aim of this study was to clarify taxonomic position of a group of 29 biochemically presumptively identified Staphylococcus strains deposited in the Czech Collection of Microorganisms (http://www.sci.muni.cz/ccm/). Biotyping was done by API Staph, API Zym commercial kits and conventional tests. More detailed characterization was performed by ribotyping with both EcoRI and HindIII restriction endonucleases, whole-cell protein analysis and the (GTG)5-PCR fingerprinting. All applied methods congruently separated most of analysed strains and assigned them as S. haemolyticus, S. equorum, S. saprophyticus subsp. saprophyticus, S. kloosii, S. cohnii subsp. cohnii and S. epidermidis species. However these methods did not provide congruent results in several strains. Ribotyping as well as protein analysis failed to unambiguously distinguish some oxidase positive S. sciuri, S. fleurettii and S. lentus strains revealing highly similar patterns; but they were clearly separated by the (GTG)5-PCR fingerprinting. EcoRI ribotype patterns revealed more species specific clusters than HindIII profiles in these strains. And finally, two pairs of analysed strains showed high similarity to each other by using all methods. They were clearly separated from all validly described staphylococcal species and probably represent new taxons of this genera. Our results show that applied methods are suitable for classification of tested Staphylococcus spp. and that their combination is a good polyphasic approach applicable for identification of staphylococci. This study was supported by projects ns. MSM0021622416 and FRVS G4 2658.

Abstract (in Czech)

Charakterizace kmenů Staphylococcus sp. pomocí metody ribotypizace, analýzy celkových buněčných proteinů a (GTG)5-PCR.

Links

• MSM0021622416, plan (intention): Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time