The main goal of this paper is to define the basic terms of researched topic and to explain the role of science and technology parks and business incubators for the development of innovative enterprise in the regions. Other objective of this study is to identify the allocation of the supportive infrastructure for innovative enterprise in the Czech Republic and to assess the perspectives of its future development especially with regard for possibilities of public support from structural funds provided through Operating Programme Industry and Enterprise and through new Operating Programme Enterprise and Innovations.