Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells.

HARNIČAROVÁ, Andrea. Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells. Online. Exp Cell Res. Elsevier, 2006, vol. 312, No 20, 4019-4035. ISSN 0014-4827. [citováno 2024-04-23]

Basic information

• Original name: Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells.
• Authors: HARNIČAROVÁ, Andrea
• Edition: Exp Cell Res. Elsevier, 2006, 0014-4827.

Other information

• Type of outcome: Article in a journal
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.777
• Organization unit: Faculty of Science
• UT WoS: 000242838100006
• Keywords in English: Chromosome structure; c-myc gene; Gene expression; RNA-FISH, Differentiation
• Tags: c-myc gene, chromosome structure, GENE EXPRESSION, RNA-FISH, Differentiation

Abstract

Using sequential RNA-DNA fluorescence in situ hybridization, the nuclear arrangement of both the active and inactive c-myc gene as well as its transcription was investigated in colon cancer HT-29 cells induced to differentiate into enterocytes. Cytogenetic studies revealed the presence of two chromosomes 8 in HT-29 cells, of which the one containing c-myc gene amplicons was substantially larger and easily distinguished from the normal chromosome. This observation enabled detection of both activity and nuclear localization of c-myc genes in single cells and in individual chromosome territories. Similar transcriptional activity of the c-myc gene was observed in both the normal and derivative chromosome 8 territories showing no influence of the amplification on the c-myc gene expression. Our experiments demonstrate strikingly specific nuclear and territorial arrangements of active genes as compared with inactive ones: on the periphery of their territories facing to the very central region of the cell nucleus. Nuclear arrangement of c-myc genes and transcripts was conserved during cell differentiation and, therefore, independent of the level of differentiation-specific c-myc gene expression. However, after the induction of differentiation, a more internal territorial location was found for the single copy c-myc gene of normal chromosome 8, while amplicons conserved their territorial topography.