Detailed Information on Publication Record
2006
Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells.
HARNIČAROVÁ, AndreaBasic information
Original name
Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells.
Authors
Edition
Exp Cell Res. Elsevier, 2006, 0014-4827
Other information
Type of outcome
Článek v odborném periodiku
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.777
Organization unit
Faculty of Science
UT WoS
000242838100006
Keywords in English
Chromosome structure; c-myc gene; Gene expression; RNA-FISH, Differentiation
Změněno: 12/12/2007 11:13, Mgr. Jiří Pacherník, Ph.D.
Abstract
V originále
Using sequential RNA-DNA fluorescence in situ hybridization, the nuclear arrangement of both the active and inactive c-myc gene as well as its transcription was investigated in colon cancer HT-29 cells induced to differentiate into enterocytes. Cytogenetic studies revealed the presence of two chromosomes 8 in HT-29 cells, of which the one containing c-myc gene amplicons was substantially larger and easily distinguished from the normal chromosome. This observation enabled detection of both activity and nuclear localization of c-myc genes in single cells and in individual chromosome territories. Similar transcriptional activity of the c-myc gene was observed in both the normal and derivative chromosome 8 territories showing no influence of the amplification on the c-myc gene expression. Our experiments demonstrate strikingly specific nuclear and territorial arrangements of active genes as compared with inactive ones: on the periphery of their territories facing to the very central region of the cell nucleus. Nuclear arrangement of c-myc genes and transcripts was conserved during cell differentiation and, therefore, independent of the level of differentiation-specific c-myc gene expression. However, after the induction of differentiation, a more internal territorial location was found for the single copy c-myc gene of normal chromosome 8, while amplicons conserved their territorial topography.