KŘÍŽKOVÁ, Soňa, Libuše TRNKOVÁ, Dalibor HÚSKA and René KIZEK. mRNA isolation by using paramagnetic particles and its electrochemical quantification as a tool for transcriptome analysis. In Genetic toxicology and cancer prevention. Bratislava, Slovak republic: Genetic toxicology and cancer prevention, 2007, p. 27-30. ISBN 978-80-970128-5-4.
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Basic information
Original name mRNA isolation by using paramagnetic particles and its electrochemical quantification as a tool for transcriptome analysis
Name in Czech Izolace mRNA použitím paramagnetických kuliček a její elektrochemická kvantifikace jako nástroj pro analýzu transkriptomu
Authors KŘÍŽKOVÁ, Soňa (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor, belonging to the institution), Dalibor HÚSKA (203 Czech Republic) and René KIZEK (203 Czech Republic).
Edition Bratislava, Slovak republic, Genetic toxicology and cancer prevention, p. 27-30, 4 pp. 2007.
Publisher Genetic toxicology and cancer prevention
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/07:00050870
Organization unit Faculty of Science
ISBN 978-80-970128-5-4
Keywords in English Transcriptom; mRNA; paramagnetic beads
Tags mRNA, paramagnetic beads, rivok, Transcriptom, ZR
Changed by Changed by: prof. RNDr. Libuše Trnková, CSc., učo 1027. Changed: 12/3/2012 10:05.
Abstract
Transcriptom - set of all mRNA molecule, "transcripts" produced in the cell can inform us about the metabolic and proliferation state of the cell and at the same time it gives us a notice about the cell response to external factors. The increase of the level of total transcriptome signalizes a changed cell state with regard to above mentioned factors. In this respect it shows to be interesting to study the transcriptome level at tumour cells. The present work reports on suggesting and optimizing of mRNA separation from biological material and its detection via paramagnetic beads coupled with electrochemical detection.
Abstract (in Czech)
Transkriptom - soubor všech mRNA molekul, "transkripty" produkované v buňce nás mohou informovat o metabolickém a proliferačních stavu buňky a ve stejné době nám vypovídá o odpovědi buňky na vnější faktory. Zvýšené množství celkového transkriptomu signalizuje o změnách stavu buňky týkajících se výše zmíněných faktorů. V tomto ohledu je velice zajímavé studovat hladinu transkriptomu u nádorových buněk. Tato práce popisuje navrhnutí a optimalizaci separace mRNA z biologického materiálu a její detekce pomocí paramagnetických kuliček spojených s elektrochemickou detekcí.
Links
LC06035, research and development projectName: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.
Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine
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