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@proceedings{746221,
author = {Garajová, Ingrid and Fabian, Pavel and Nenutil, Rudolf and Slabý, Ondřej and Kocáková, Ilona and Grell, Peter and Hanzelková, Zina and Svoboda, Marek and Vyzula, Rostislav},
booktitle = {Abstract book.},
keywords = {regulatory T-cells; colon carcinoma},
language = {eng},
title = {Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma},
year = {2007}
}
TY - CONF
ID - 746221
AU - Garajová, Ingrid - Fabian, Pavel - Nenutil, Rudolf - Slabý, Ondřej - Kocáková, Ilona - Grell, Peter - Hanzelková, Zina - Svoboda, Marek - Vyzula, Rostislav
PY - 2007
TI - Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma
KW - regulatory T-cells
KW - colon carcinoma
N2 - We have previously shown that colon carcinoma (CC) is infiltrated by FOXP3-positive lymphocytes. Although the transcription factor FOXP3 is claimed to be specific for regulatory T (TREG) cells, the canonical TREG population is known to show the CD4+ CD25+ (FOXP3+) immunophenotype. The aim of the current study was therefore to investigate whether FOXP3+ cells infiltrating CC coexpress indeed CD4 and CD25 molecules. Double-staining experiments were therefore performed on serial sections obtained from formalin-fixed, paraffin-embedded specimens of nine cases of CC. The CC infiltrate included numerous CD4+ as well as CD25+ cells; moreover CD4+ cells harboured both FOXP3+ and FOXP3- lymphocytes; similarly, CD25+ cells included both FOXP3+ and FOXP3- lymphocytes. Nevertheless, all the FOXP3+ cells showed simultaneously CD4-positivity; similarly, all the FOXP3+ cells showed simultaneously CD25-positivity. Thus, the FOXP3+ lymphocytes infiltrating CC are characterized by the coexpression of both CD4 and CD25 molecules, and show therefore an immunophenotype overlapping with that of standard CD4+CD25+FOXP3+ TREG cells. The presence of lymphocytes resembling TREG cells within the microenvironment of CC can explain why the CC tumour mass, despite being also infiltrated by cytolytic anti-tumour CD8+/CD4+ lymphocytes, shows however the well known capability to undergo fatal progression. Acknowledgment: This project is supported by Internal Grant Agency, Ministry of Health, Czech Republic. No.: NR/9076-4.
ER -
GARAJOVÁ, Ingrid, Pavel FABIAN, Rudolf NENUTIL, Ondřej SLABÝ, Ilona KOCÁKOVÁ, Peter GRELL, Zina HANZELKOVÁ, Marek SVOBODA and Rostislav VYZULA. Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma. In \textit{Abstract book.}. 2007.
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