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@article{746497,
author = {Maleková, Lubica and Tomášková, Jana and Nováková, Marie and Štefánik, Peter and Kopáček, Juraj and Lakatoš, Boris and Pastoreková, Silvia and Križanová, Olga and Breier, Albert and Ondriaš, Karol},
article_location = {Ulm},
article_number = {2},
keywords = {Mitochondria;cardiac myocytes;chloride channels;chloride conductance;apoptosis;single channel},
language = {eng},
issn = {0031-6768},
journal = {Pflügers Archiv - Eur.J. Physiol.},
title = {Inhibitory effect of DIDS, NPPB, and phloretin on intracellular chloride channels},
volume = {455},
year = {2007}
}
TY - JOUR
ID - 746497
AU - Maleková, Lubica - Tomášková, Jana - Nováková, Marie - Štefánik, Peter - Kopáček, Juraj - Lakatoš, Boris - Pastoreková, Silvia - Križanová, Olga - Breier, Albert - Ondriaš, Karol
PY - 2007
TI - Inhibitory effect of DIDS, NPPB, and phloretin on intracellular chloride channels
JF - Pflügers Archiv - Eur.J. Physiol.
VL - 455
IS - 2
SP - 349–357
EP - 349–357
PB - Springer
SN - 00316768
KW - Mitochondria;cardiac myocytes;chloride channels;chloride conductance;apoptosis;single channel
N2 - We studied the effects of the chloride channel blockers, 5-nitro-2-(phenylpropylamino)-benzoate (NPPB), dihydro-4,4 diisothiocyanostilbene-2,2-disulphonic acid (DIDS), and phloretin on H2O2-induced primary culture cardiomyocyte apoptosis and activity of intracellular chloride channels obtained from rat heart mitochondrial and lysosomal vesicles. The chloride channel blockers (100 micromol/l) inhibited the H2O2-induced cardiomyocytes apoptosis. We characterized the effect of the blockers on single channel properties of the chloride channels derived from the mitochondrial and lysosomal vesicles incorporated into a bilayer lipid membrane. The single chloride channel currents were measured in 250:50 mmol/l KCl cis/trans solutions. NPPB, DIDS, and phloretin inhibited the chloride channels by decreasing the channel open probability in a concentration-dependent manner with EC50 values of 42, 7, and 20 micromol/l, respectively. NPPB and phloretin inhibited the channels conductance and open dwell time, indicating that they could affect the chloride selective filter, pore permeability, and gating mechanism of the chloride channels. DIDS and NPPB inhibited the channels from the other side than bongkrekic acid and carboxyatractyloside. The results may contribute to understand a possible involvement of intracellular chloride channels in apoptosis and cardioprotection.
ER -
MALEKOVÁ, Lubica, Jana TOMÁŠKOVÁ, Marie NOVÁKOVÁ, Peter ŠTEFÁNIK, Juraj KOPÁČEK, Boris LAKATOŠ, Silvia PASTOREKOVÁ, Olga KRIŽANOVÁ, Albert BREIER and Karol ONDRIAŠ. Inhibitory effect of DIDS, NPPB, and phloretin on intracellular chloride channels. \textit{Pflügers Archiv - Eur.J. Physiol.}. Ulm: Springer, 2007, vol.~455, No~2, p.~349–357. ISSN~0031-6768.
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