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@inproceedings{746854,
author = {Adam, Vojtěch and Diopan, Václav and Beklová, Miroslava and Horna, Aleš and Zeman, Ladislav and Trnková, Libuše and Kizek, René},
address = {Univerzita Pardubice},
booktitle = {Vitamins 2007- Nutrition and Diagnostics},
keywords = {Coulometric detection; nucleic acids; liquid chromatography},
language = {eng},
location = {Univerzita Pardubice},
isbn = {978-80-7194-937-4},
pages = {96-97},
publisher = {Univerzita Pardubice},
title = {Application of coulometric detection to the molecular biochemistry},
year = {2007}
}
TY - JOUR
ID - 746854
AU - Adam, Vojtěch - Diopan, Václav - Beklová, Miroslava - Horna, Aleš - Zeman, Ladislav - Trnková, Libuše - Kizek, René
PY - 2007
TI - Application of coulometric detection to the molecular biochemistry
PB - Univerzita Pardubice
CY - Univerzita Pardubice
SN - 9788071949374
KW - Coulometric detection
KW - nucleic acids
KW - liquid chromatography
N2 - In the present work, we aimed on optimizing liquid chromatography coupled with twelve channel coulometric detector to detect markers of oxidative damaging of nucleic acids. Primarily, we aimed on investigating of influence of fundamental experimental conditions such as composition of mobile phase and its flow rate, column temperature and working electrodes potential on current responses of 8-hydroxyguanosine. The optimized conditions were as follows -- mobile phase: acetate buffer with flow rate of 0.5 ml.min-1, working electrodes potential: -400 mV, column temperature: 30 degree of C. The detection limit (3 S/N) was evaluated as tens of femtomoles per injection. Based on the results obtained the technique could be used for analysis of clinical samples.
ER -
ADAM, Vojtěch, Václav DIOPAN, Miroslava BEKLOVÁ, Aleš HORNA, Ladislav ZEMAN, Libuše TRNKOVÁ and René KIZEK. Application of coulometric detection to the molecular biochemistry. In \textit{Vitamins 2007- Nutrition and Diagnostics}. Univerzita Pardubice: Univerzita Pardubice, 2007, p.~96-97. ISBN~978-80-7194-937-4.
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