Coupling of monolithic column sample preparation and flow
injection analysis - Electrochemical detection for
determination of lactoferrin: easy, low cost and structural
distuinguishing

D 2007

Coupling of monolithic column sample preparation and flow injection analysis - Electrochemical detection for determination of lactoferrin: easy, low cost and structural distuinguishing

HORNA, Aleš, Ondřej ZÍTKA, Soňa KŘÍŽKOVÁ, Vendula HRDINOVÁ, Johana NÁKIELNÁ et. al.

Basic information

Original name

Coupling of monolithic column sample preparation and flow injection analysis - Electrochemical detection for determination of lactoferrin: easy, low cost and structural distuinguishing

Name in Czech

Spojení přípravy vzorků na monolithické koloně a průtokové injekční analyzy - elektrochemické detekce pro stanovení laktoferinu: jednoduché, nenákladné a strukturně poznávací

Authors

HORNA, Aleš, Ondřej ZÍTKA, Soňa KŘÍŽKOVÁ, Vendula HRDINOVÁ, Johana NÁKIELNÁ, Vojtěch ADAM, Libuše TRNKOVÁ, Ladislav ZEMAN and René KIZEK

Edition

Belgické království, 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007, p. 775-775, 1 pp. 2007

Publisher

31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

Belgium

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Keywords in English

Lactoferrin; flow ingection analysis; electrochemical detection

Abstract

ORIG CZ

V originále

Lactoferrin is a glyco- and iron-binding protein consisted from 703 aminoacid residues. Its molecular weight is of about 77 000 Da. Except iron lactoferrin is able to bind other heavy metals into the same binding place, but with lower affinity. The main biological function of lactoferrin is to maintain iron homeostasis. Iron capturing occurs primary in the intestine from food. It is related to cell growth regulation, cell differentiation and it has an antiinflammatory activity. The main aim of this work was to suggest a method to distinguish structure of lactoferrin easily and with low demanding on instruments and costs. As detection part of the method we used flow injection analysis with electrochemical detection.

In Czech

Laktoferin je glyko- a železo-vázající protein sestávající ze 703 aminokyselinových zbytků. Jeho molekulová hmotnost je asi 77 kDa. Kromě železa je laktoferin schopen vázat i jiné těžké kovy na stejné vazebné místo, ale s nižší afinitou. Jeho hlavní biologická funkce je udržování homeostáze železa. Vychytávání železa z potravy se děje především ve střevech. Je spojován s regulací buněčného růstu, buněčnou diferenciací a vykazuje protizánětlivou aktivitu. Hlavním cílem této práce bylo navrhnout jednoduchou, nenákladnou metodu pro zkoumání struktury laktoferinu. Jako detekční část metody jsme pouzili průtokovou injekční analýzu s elektrochemickou detekcí.

Citovat

HORNA, Aleš, Ondřej ZÍTKA, Soňa KŘÍŽKOVÁ, Vendula HRDINOVÁ, Johana NÁKIELNÁ, Vojtěch ADAM, Libuše TRNKOVÁ, Ladislav ZEMAN and René KIZEK. Coupling of monolithic column sample preparation and flow injection analysis - Electrochemical detection for determination of lactoferrin: easy, low cost and structural distuinguishing. In 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007. Belgické království: 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007, 2007, p. 775-775.

@inproceedings{746856,
   author = {Horna, Aleš and Zítka, Ondřej and Křížková, Soňa and Hrdinová, Vendula and Nákielná, Johana and Adam, Vojtěch and Trnková, Libuše and Zeman, Ladislav and Kizek, René},
   address = {Belgické království},
   booktitle = {31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007},
   keywords = {Lactoferrin; flow ingection analysis; electrochemical detection},
   language = {eng},
   location = {Belgické království},
   pages = {775-775},
   publisher = {31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007},
   title = {Coupling of monolithic column sample preparation and flow injection analysis - Electrochemical detection for determination of lactoferrin: easy, low cost and structural distuinguishing},
   year = {2007}
}

TY  - JOUR
ID  - 746856
AU  - Horna, Aleš - Zítka, Ondřej - Křížková, Soňa - Hrdinová, Vendula - Nákielná, Johana - Adam, Vojtěch - Trnková, Libuše - Zeman, Ladislav - Kizek, René
PY  - 2007
TI  - Coupling of monolithic column sample preparation and flow injection analysis - Electrochemical detection for determination of lactoferrin: easy, low cost and structural distuinguishing
PB  - 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007
CY  - Belgické království
KW  - Lactoferrin
KW  - flow ingection analysis
KW  - electrochemical detection
N2  - Lactoferrin is a glyco- and iron-binding protein consisted from 703 aminoacid residues. Its molecular weight is of about 77 000 Da. Except iron lactoferrin is able to bind other heavy metals into the same binding place, but with lower affinity. The main biological function of lactoferrin is to maintain iron homeostasis. Iron capturing occurs primary in the intestine from food. It is related to cell growth regulation, cell differentiation and it has an antiinflammatory activity. The main aim of this work was to suggest a method to distinguish structure of lactoferrin easily and with low demanding on instruments and costs. As detection part of the method we used flow injection analysis with electrochemical detection.
ER  -

HORNA, Aleš, Ondřej ZÍTKA, Soňa KŘÍŽKOVÁ, Vendula HRDINOVÁ, Johana NÁKIELNÁ, Vojtěch ADAM, Libuše TRNKOVÁ, Ladislav ZEMAN and René KIZEK. Coupling of monolithic column sample preparation and flow injection analysis - Electrochemical detection for determination of lactoferrin: easy, low cost and structural distuinguishing. In \textit{31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007}. Belgické království: 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007, 2007, p.~775-775.

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