Determination of isoflavones using liquid chromatography with electrochemical detection

MIKELOVÁ, Radka, Petr HODEK, Pavel HANUŠTIAK, Vojtěch ADAM, Soňa KŘÍŽKOVÁ, Ladislav HAVEL, Marie STIBOROVÁ, Aleš HORNA, Miroslava BEKLOVÁ, Libuše TRNKOVÁ and René KIZEK. Determination of isoflavones using liquid chromatography with electrochemical detection. Acta Chimica Slovenica. 2007, vol. 54, No 1, p. 92-97. ISSN 1318-0207.

Basic information

• Original name: Determination of isoflavones using liquid chromatography with electrochemical detection
• Name in Czech: Stanovení izoflavonů použitím kapalinové chromatografie s elektrochemickou detekcí
• Name (in English): Determination of isoflavones using liquid chromatography with electrochemical detection
• Authors: MIKELOVÁ, Radka (203 Czech Republic, guarantor, belonging to the institution), Petr HODEK (203 Czech Republic), Pavel HANUŠTIAK (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Soňa KŘÍŽKOVÁ (203 Czech Republic), Ladislav HAVEL (203 Czech Republic), Marie STIBOROVÁ (203 Czech Republic, belonging to the institution), Aleš HORNA (203 Czech Republic), Miroslava BEKLOVÁ (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution) and René KIZEK (203 Czech Republic).
• Edition: Acta Chimica Slovenica, 2007, 1318-0207.

Other information

• Original language: Czech
• Type of outcome: Article in a journal
• Field of Study: 10405 Electrochemistry
• Country of publisher: Slovenia
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 1.093
• RIV identification code: RIV/00216224:14310/07:00050876
• Organization unit: Faculty of Science
• UT WoS: 000245178800014
• Keywords in English: Isoflavonoids; injection flow analysis; HPLC
• Tags: HPLC, injection flow analysis, Isoflavonoids, rivok, ZR

Abstract

Mezi významné biologické role izoflavonů patří také jejich vliv na karcinogenezi. Použili jsme injekční průtokovou analýzu a HPLC s elektrochemickou detekcí pro současné stanovení různých izoflavonů (biochanin A, formononetin,sissotrin, daidzin, daidzein, glycitin, glycitein and genistein). Nejvhodnější chromatografické podmínky byly: mobilní fáze: 0,2 mol L-1 acetátový pufr (pH 5,0); rychlost průtoku 2,0 mL min-1;teplota kolony a detektoru: 26 st. C; detekční potenciál: 800 mV. Podle těchto optimálních podmínek byl limit detekce v ng mL-1. Jejich současná detekce trvala 15 minut.

Abstract (in English)

Among the biologically important roles of isoflavones is also their effect on carcinogenesis. We used flow injection analysis and high performance liquid W with electrochemical detection to simultaneously determine certain isoflavones (biochanin A, formononetin, sissotrin, daidzin, daidzein, glycitin, glycitein and genistein). The most suitable chromatographic conditions were: mobile phase: 0.2 mol L-1 acetate buffer (pH 5.0); flow rate 2.0 mL min-1; column and detector temperature: 26 dg. C; detection potential: 800 mV. Under the optimal conditions, the detection limits were in the range of several ng mL-1. Their simultaneous determination takes 15 min.

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)