Easy and rapid separation and determination of mRNA from a
biological sample by means of paramagnetic beads coupled with
electrochemical detection

D 2007

Easy and rapid separation and determination of mRNA from a biological sample by means of paramagnetic beads coupled with electrochemical detection

HÚSKA, Dalibor, Soňa KŘÍŽKOVÁ, Jiří BALOUN, Vojtěch ADAM, Aleš HORNA et. al.

Basic information

Original name

Easy and rapid separation and determination of mRNA from a biological sample by means of paramagnetic beads coupled with electrochemical detection

Name in Czech

Jednoduchá a rychlá separace a stanovení mRNA z biologických vzorků pomocí paramagnetických kuliček spojené s elektrochemickou detekcí

Authors

HÚSKA, Dalibor, Soňa KŘÍŽKOVÁ, Jiří BALOUN, Vojtěch ADAM, Aleš HORNA, Libuše TRNKOVÁ and René KIZEK

Edition

Litevská republika, 4th Nordic Separation Science Society (NoSSS) International Conference, p. 79-79, 1 pp. 2007

Publisher

4th Nordic Separation Science Society (NoSSS) International Conference

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Lithuania

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

ISBN

978-9955-12-232-6

Keywords in English

Paramagnetic beads; mRNA; electrochemical detection

Abstract

ORIG CZ

V originále

Easy, efficient and low demanding separation of mRNA from biological material is needed to study gene expression and to use in chip technologies. To rapid and selective isolation of mRNA new approaches using modified surface of paramagnetic beads have been developed. It is common knowledge that each mRNA molecule contains sequence of 25 adenines. This feature can be used for binding of mRNA on the surface of the beads coated by thymine chains. The present work reports on suggesting and optimizing of mRNA separation and detection from biological material via paramagnetic beads coupled with electrochemical detection.

In Czech

Jednochá, účinná a nenáročná separace mRNA z bolofického materiálu je potřeba pro studium genové exprese a použití v čipových technologiích. Pro rychlou a selektivní izolaci mRNA byly vyvinuty nové přístupy s použitím paramagnetických kuliček s modifikovaným povrchem. Je známo, že každá molekula mRNA obsahuje sekvenci 25 adeninů, tato vlastnost může být použita pro navázání mRNA na povrch kuliček pokrytých řetězci tyminů. V této práci byla navrhnuta a optimalizována mRNA separace pomocí paramagnetických kuliček s elektrochemickou detekcí.

Citovat

HÚSKA, Dalibor, Soňa KŘÍŽKOVÁ, Jiří BALOUN, Vojtěch ADAM, Aleš HORNA, Libuše TRNKOVÁ and René KIZEK. Easy and rapid separation and determination of mRNA from a biological sample by means of paramagnetic beads coupled with electrochemical detection. In 4th Nordic Separation Science Society (NoSSS) International Conference. Litevská republika: 4th Nordic Separation Science Society (NoSSS) International Conference, 2007, p. 79-79. ISBN 978-9955-12-232-6.

@inproceedings{746859,
   author = {Húska, Dalibor and Křížková, Soňa and Baloun, Jiří and Adam, Vojtěch and Horna, Aleš and Trnková, Libuše and Kizek, René},
   address = {Litevská republika},
   booktitle = {4th Nordic Separation Science Society (NoSSS) International Conference},
   keywords = {Paramagnetic beads; mRNA; electrochemical detection},
   language = {eng},
   location = {Litevská republika},
   isbn = {978-9955-12-232-6},
   pages = {79-79},
   publisher = {4th Nordic Separation Science Society (NoSSS) International Conference},
   title = {Easy and rapid separation and determination of mRNA from a biological sample by means of paramagnetic beads coupled with electrochemical detection},
   year = {2007}
}

TY  - JOUR
ID  - 746859
AU  - Húska, Dalibor - Křížková, Soňa - Baloun, Jiří - Adam, Vojtěch - Horna, Aleš - Trnková, Libuše - Kizek, René
PY  - 2007
TI  - Easy and rapid separation and determination of mRNA from a biological sample by means of paramagnetic beads coupled with electrochemical detection
PB  - 4th Nordic Separation Science Society (NoSSS) International Conference
CY  - Litevská republika
SN  - 9789955122326
KW  - Paramagnetic beads
KW  - mRNA
KW  - electrochemical detection
N2  - Easy, efficient and low demanding separation of mRNA from biological material is needed to study gene expression and to use in chip technologies. To rapid and selective isolation of mRNA new approaches using modified surface of paramagnetic beads have been developed. It is common knowledge that each mRNA molecule contains sequence of 25 adenines. This feature can be used for binding of mRNA on the surface of the beads coated by thymine chains. The present work reports on suggesting and optimizing of mRNA separation and detection from biological material via paramagnetic beads coupled with electrochemical detection.
ER  -

HÚSKA, Dalibor, Soňa KŘÍŽKOVÁ, Jiří BALOUN, Vojtěch ADAM, Aleš HORNA, Libuše TRNKOVÁ and René KIZEK. Easy and rapid separation and determination of mRNA from a biological sample by means of paramagnetic beads coupled with electrochemical detection. In \textit{4th Nordic Separation Science Society (NoSSS) International Conference}. Litevská republika: 4th Nordic Separation Science Society (NoSSS) International Conference, 2007, p.~79-79. ISBN~978-9955-12-232-6.

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