A new electrochemical assay for determination of phytochelatin
synthase activity

D 2007

A new electrochemical assay for determination of phytochelatin synthase activity

HORNA, Aleš, Veronika ŠUPÁLKOVÁ, Karel STEJSKAL, Jiří BALOUN, Jitka PETRLOVÁ et. al.

Basic information

Original name

A new electrochemical assay for determination of phytochelatin synthase activity

Name in Czech

Nová elektrochemická analýza pro stanovení aktivity fytochelatin syntázy

Authors

HORNA, Aleš, Veronika ŠUPÁLKOVÁ, Karel STEJSKAL, Jiří BALOUN, Jitka PETRLOVÁ, Vojtěch ADAM, Ladislav HAVEL, Libuše TRNKOVÁ, René KIZEK and Josef ZEHNÁLEK

Edition

Belgické království, 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007, p. 911-911, 1 pp. 2007

Publisher

31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

Belgium

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Keywords in English

Phytochelatin synthase; electrochemistry

Abstract

ORIG CZ

V originále

Plants respond to heavy metal toxicity in a variety of different ways such as immobilization, exclusion, chelation and compartmentalization of the metal ions, and the expression of more general stress response mechanisms e.g. ethylene and stress peptides. One of the most studied plant stress peptides are phytochelatins participating in the detoxification of heavy metals. The synthesis of phytochelatins is catalyzed by phytochelatin synthase (PCS). The possible way of the study of PCS activity in the plant cell exposed by heavy metal is determination of increasing phytochelatins concentration. The main aim of the present work was to suggest a new tool (high performance liquid chromatography coupled with twelve channel electrochemical detector) for the determination of the phytochelatin synthase activity.

In Czech

Rostiny reagují na intoxikaci těžkými kovy různými způsoby jako jsou, imobilizace, vylučování, chelatací a kompartmentalizací iontů kovů, a expresí více obecných stresových responzivních mechanismů např. etylen a stresové peptidy. Jednimi z nejvíce studovaných rostliných stresových peptidů jsou fytochelatiny, kteří se podílejí na detoxikaci těžkých kovů. Syntéza fytochelatinů je katalyzována fytochelatin syntázou (PCS). Možný způsob studia PCS aktivity v rostlinných buňkách exponovaných těžkým kovům je stanovení zvýšené koncentrace fytochelatinů. Hlavním cílem této práce bylo navrhnout nový nástroj (HPLC s dvanáctikanálovým elektrochemickým detektorem) pro stanovení fytochelatin syntázové aktivity.

Citovat

HORNA, Aleš, Veronika ŠUPÁLKOVÁ, Karel STEJSKAL, Jiří BALOUN, Jitka PETRLOVÁ, Vojtěch ADAM, Ladislav HAVEL, Libuše TRNKOVÁ, René KIZEK and Josef ZEHNÁLEK. A new electrochemical assay for determination of phytochelatin synthase activity. In 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007. Belgické království: 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007, 2007, p. 911-911.

@inproceedings{746865,
   author = {Horna, Aleš and Šupálková, Veronika and Stejskal, Karel and Baloun, Jiří and Petrlová, Jitka and Adam, Vojtěch and Havel, Ladislav and Trnková, Libuše and Kizek, René and Zehnálek, Josef},
   address = {Belgické království},
   booktitle = {31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007},
   keywords = {Phytochelatin synthase; electrochemistry},
   language = {eng},
   location = {Belgické království},
   pages = {911-911},
   publisher = {31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007},
   title = {A new electrochemical assay for determination of phytochelatin synthase activity},
   year = {2007}
}

TY  - JOUR
ID  - 746865
AU  - Horna, Aleš - Šupálková, Veronika - Stejskal, Karel - Baloun, Jiří - Petrlová, Jitka - Adam, Vojtěch - Havel, Ladislav - Trnková, Libuše - Kizek, René - Zehnálek, Josef
PY  - 2007
TI  - A new electrochemical assay for determination of phytochelatin synthase activity
PB  - 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007
CY  - Belgické království
KW  - Phytochelatin synthase
KW  - electrochemistry
N2  - Plants respond to heavy metal toxicity in a variety of different ways such as immobilization, exclusion, chelation and compartmentalization of the metal ions, and the expression of more general stress response mechanisms e.g. ethylene and stress peptides. One of the most studied plant stress peptides are phytochelatins participating in the detoxification of heavy metals. The synthesis of phytochelatins is catalyzed by phytochelatin synthase (PCS). The possible way of the study of PCS activity in the plant cell exposed by heavy metal is determination of increasing phytochelatins concentration. The main aim of the present work was to suggest a new tool (high performance liquid chromatography coupled with twelve channel electrochemical detector) for the determination of the phytochelatin synthase activity.
ER  -

HORNA, Aleš, Veronika ŠUPÁLKOVÁ, Karel STEJSKAL, Jiří BALOUN, Jitka PETRLOVÁ, Vojtěch ADAM, Ladislav HAVEL, Libuše TRNKOVÁ, René KIZEK and Josef ZEHNÁLEK. A new electrochemical assay for determination of phytochelatin synthase activity. In \textit{31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007}. Belgické království: 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007, 2007, p.~911-911.

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