HAVELKOVÁ, Marie and Inge HONES. THE EFFECT OF MATING FACTOR ON YARROWIA LIPOLYTICA. 1st ed. Francie, Paříž: Univ. L. Pasteur, Paris, Prance, 2007, 7 pp. 9th Annual Conference on Yarrowia lipolytica.
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Basic information
Original name THE EFFECT OF MATING FACTOR ON YARROWIA LIPOLYTICA
Name in Czech VLIV MATING FAKTORU NA BUŇKY YARROWIA LIPOLYTICA.
Authors HAVELKOVÁ, Marie (203 Czech Republic, guarantor) and Inge HONES (276 Germany).
Edition 1. vyd. Francie, Paříž, 7 pp. 9th Annual Conference on Yarrowia lipolytica. 2007.
Publisher Univ. L. Pasteur, Paris, Prance
Other information
Original language English
Type of outcome Special-purpose publication
Field of Study Genetics and molecular biology
Country of publisher France
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14410/07:00032805
Organization unit Faculty of Education
Keywords in English Cells cells type A cells type B haploid cells cell division G1 phase inhibition cell culture synchonization
Tags International impact
Changed by Changed by: doc. MUDr. Marie Havelková, CSc., učo 1564. Changed: 10/7/2009 13:37.
Abstract
Yarrowia lipolytica has two mating types designated A and B. Mating type A cells produce mating specific pheromone (A factor). Crude fractions of the A factor was partially purified and concentrated (1.000 fold) by Amberlite CG 50. This A factor acts only on cells of opposite mating type inducning a cascade of metabolic and morphological changes. Ths A factor specially inhibits the cell division of mating type B. The cell were arrested in the G1 phase (no budding, characterized by light microscopy). The arrest is reversible and the lenght of the arrest period is dependent on the concentration of A factor added. The A factor is one element of a relatory system whose functions assure the synchronization of haploid cells for conjugation. In an asynchronous population only a small part of cells are in this specific stage. When A factor is added to conjugation medium (YM), the time for conjugation was shorter and the conjugation rate was increased.
Abstract (in Czech)
Yarrowia lipolytica existuje v buňkách dvou typů, které byly označeny jako buňky A a buňky B. Buňky mating typu A produkují specifický mating feromon (A faktor). Tento faktor vyvolává u buněk opačného mating typu (buněk B)řadu kaskádových metabolických a v důsledku toho i morfologických změn. Mj. blokuje dělení těchto buněk. Buňky jsou blokovány v G1 fázi buněčného cyklu. Inhibice je reversibilní a délka jejího trvání závisí na koncentraci přidaného A faktoru. Zjistili jsme, že A faktor lze s výhodou použít k synchronizaci buněčné kultury haploidních buněk B typu. S takovou kulturou buněk je pak velmi výhodné provádět různé typy pokusů.
Links
MSM0021622421, plan (intention)Name: Škola a zdraví pro 21. století
Investor: Ministry of Education, Youth and Sports of the CR, School and Health for the 21st Century
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