AVRANAS, Antonis, Sofia KOURTIDU and Vladimír VETTERL. Adsorption of oligonucleotides A10, A25, A50 and A80 at the mercury/electrolyte interface. Colloids and Surfaces A: Physicochemical and Engineering Aspects. Amsterdam: Elsevier, 2007, vol. 295, March, p. 178-184. ISSN 0927-7765.
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Basic information
Original name Adsorption of oligonucleotides A10, A25, A50 and A80 at the mercury/electrolyte interface.
Name in Czech Adsorpce oligonukleotidů A10, A25, A50 a A80 na rozhraní rtuť/elektrolyt.
Authors AVRANAS, Antonis (300 Greece), Sofia KOURTIDU (300 Greece) and Vladimír VETTERL (203 Czech Republic, guarantor).
Edition Colloids and Surfaces A: Physicochemical and Engineering Aspects, Amsterdam, Elsevier, 2007, 0927-7765.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10610 Biophysics
Country of publisher Greece
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.109
RIV identification code RIV/00216224:14110/07:00019572
Organization unit Faculty of Medicine
UT WoS 000244737700028
Keywords in English Oligonucleotides; A10; A25; A50; A80 ; differential capacitance; adsorption;
Tags A10, A25, A50, A80, adsorption, differential capacitance, Oligonucleotides
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Vladimír Vetterl, DrSc., učo 1954. Changed: 17/1/2008 16:39.
Abstract
The adsorption of the oligonucleotides A10, A25, A50 and A80 at the mercury electrolyte solution interface is studied. The oligonucleotides are adsorbed at a potential close to -0.5 V (versus Ag/AgCl reference electrode) but they are desorbed at one or two potential ranges depending on the number of adenine molecules. The effect of the waiting time at the potential of maximum adsorption is studied and this time is closely related to the desorption peak heights. The adsorption/desorption is studied at two oligonucleotide concentrations and at two temperatures. The potentials of the desorption peaks of all oligonucleotides studied have almost the same value, indicating that the desorption process is almost the same. Capacity time curves following potential jumps from the adsorption region to desorption potentials show that the desorption is a very fast process but is usually followed by a reorientation/readsorption of the molecules at the interface depending on the number of adenines.
Abstract (in Czech)
Je studována adsorpce oligonukleotidů A10, A25, A50 a A80 na rozhraní mezi rtutí a roztokem elektrolytu. Oligonukleotidy jsou adsorbovány v okolí potwenciálu -0,5 V (oproti Ag/AgCl referenční elelktrodě), ale jsou desorbovány v jednom nebo dvou rozsazích potenciálů v závislosti na délce oligonukleotidu. Je studován vliv čekací doby při potenciálu maximální adsorpce. Bylo zjištěno, že výška desorpčních píků závisí na tomto čekacím čase. Adsorpčně-desorpční děj byl studován při dvou koncentracích oligonukleotidů a dvou teplotách. Potenciály desorpčních píků všech studovaných oligonukleotidů byly téměř stejné. Po skoku potenciálu z oblasti adsorpce do oblasti desorpce byla měřena závislost diferenciální kapacity C na čase t. Z C-t křivek lze usoudit, že desoprce je velmi rychlý děj, po kterém obvykle následuje reorientace/readsorpce molekul v závislosti na délce oligonukleotidu.
Links
GA203/07/1195, research and development projectName: Analýza struktury a interakcí DNA pomocí elektrochemických technik a chemických sond. Nové metody a senzory pro detekci poškození DNA
IAA4004702, research and development projectName: Adsorpce bazí, nukleosidů, nukleotidů a polynukleotidů na elektrodách
Investor: Academy of Sciences of the Czech Republic, Adsorption of bases, nucleosides, nucleotides and polynucleotides on electrods
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