Detailed Information on Publication Record
2007
CE separation of proteins and yeasts dynamically modified by PEG pyrenebutanoate with fluorescence detection.
HORKÁ, Marie, Filip RŮŽIČKA, Veronika HOLÁ and Karel ŠLAISBasic information
Original name
CE separation of proteins and yeasts dynamically modified by PEG pyrenebutanoate with fluorescence detection.
Name in Czech
CE separace proteinů a kvasinek, dynamicky modifikovaných PEG pyrenebutanoátem, s fluorescenční detekcí
Authors
HORKÁ, Marie (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, guarantor), Veronika HOLÁ (203 Czech Republic) and Karel ŠLAIS (203 Czech Republic)
Edition
Electrophoresis, Germany, WILEY-VCH Verlag GmbH&Co. KGaA, 2007, 0173-0835
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.609
RIV identification code
RIV/00216224:14110/07:00024145
Organization unit
Faculty of Medicine
UT WoS
000248190400022
Keywords in English
capillary isoelectric focusing; capillary electrophoresis; separation; proteins; yeasts; pyrenebutanoate
Tags
Tags
International impact, Reviewed
Změněno: 25/6/2009 10:13, prof. MUDr. Filip Růžička, Ph.D.
V originále
The optimized protocols of the bioanalytes separation, proteins and yeasts, dynamically modified by the nonionogenic tenside PEG pyrenebutanoate, were applied in CZE and CIEF with the acidic gradient in pH range 2-5.5, both with fluorescence detection. PEG pyrenebutanoate was used as a buffer additive for a dynamic modification of proteins and/or yeast samples. The narrow peaks of modified analytes were detected. The values of the pI's of the labeled proteins were calculated using new fluorescent pI markers in CIEF and they were found to be comparable with pI's of the native compounds. As an example of the possible use of the suggested CIEF technique, the mixed cultures of yeasts, Candida albicans, Candida glabrata, Candida kefyr, Candida krusei, Candida lusitaniae, Candida parapsilosis, Candida tropicalis, Candida zeylanoides, Geotrichum candidum, Saccharomyces cerevisiae, Trichosporon asahii and Yarrowia lipolytica, were reproducibly focused and separated with high sensitivity. Using UV excitation for the on-column fluorometric detection, the minimum detectable amounts of analytes, femtograms of proteins and down to ten cells injected on the separation capillary, were estimated.
In Czech
Optimalizované protokoly separace bioanalytů, proteinů a kvasinek, dynamicky upravených neionogením tenziden pyren-máselnou kyselinou, byly použity při CZE a CIEF s kyselým gradientem v pH oblasti 2 - 5.5. Použití těchto technik bylo s úspěchem ověřeno na separaci a identifikaci jednotlivých mikrobů ze směsných kultur kvasinek (Candida albicans, Candida glabrata, Candida kefyr, Candida krusei, Candida lusitaniae, Candida parapsilosis, Candida tropicalis, Candida zeylanoides, Geotrichum candidum, Saccharomyces cerevisiae, Trichosporon asahii and Yarrowia lipolytica). Používání UV excitace pro on-column fluorimetrickou detekci umožňuje detekovat jednotlivé kvasinkové buňky i minimální množství analytu (femtogramy).
Links
| IAAX00310701, research and development project |
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