Sensitive detection of oligonucleotides and their adsorption on
amalgam and titanium surface.

J 2007

Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface.

VETTERL, Vladimír, Jiří VANĚK, Stanislav HASOŇ and Raimo SILVENNOINEN

Basic information

Original name

Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface.

Name in Czech

Citlivá detekce oligonucleotidů a jejich adsorpce na povrchu amalgámu a titánu.

Authors

VETTERL, Vladimír, Jiří VANĚK, Stanislav HASOŇ and Raimo SILVENNOINEN

Edition

European Biophysics Journal, Berlin Heidelberg, Springer-Verlag, 2007, 1432-1017

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.238

Organization unit

Faculty of Medicine

Keywords in English

amalgam electrodes; oligodeoxynucleotides detection

Abstract

ORIG CZ

V originále

The application of a gold amalgam-alloy electrode (AuAE) for a sensitive voltammetric detection of different oligodeoxynucleotides (ODNs) containing the purine units within the ODN-chains in the presence of copper ions is described. Our result shows that the sensitive detection of different ODNs can be also performed at the platinum amalgam-alloy electrode (PtAE) contrary to a low sensitivity at the silver amalgam-alloy electrode (AgAE).Electrochemical impedance spectroscopy (EIS), voltammetry, AFM, and DOE was used for the investigations of adsorption of oligonucleotides on the titanium surface and the effect of mechanical (polished, etched and/or gritted surface) and chemical modification of titanium surface was studied. From the EIS, voltammetric and optical measurements the conclusions of the effect of solvent and physical or chemical modification of titanium surface on the purine (AAG)12 and pyrimidine (TTC)12 oligonucleotide adsorption was delivered.

In Czech

Je popsáno použití amalgamových elektrod k citlivé detekci oligodeoxynukleotidů.

Links

1M0528, research and development project

Name: Stomatologické výzkumné centrum

Investor: Ministry of Education, Youth and Sports of the CR, Dental research centre

Citovat

VETTERL, Vladimír, Jiří VANĚK, Stanislav HASOŇ and Raimo SILVENNOINEN. Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface. European Biophysics Journal. Berlin Heidelberg: Springer-Verlag, 2007, vol. 36, No 2007, p. 204. ISSN 1432-1017.

@article{749694,
   author = {Vetterl, Vladimír and Vaněk, Jiří and Hasoň, Stanislav and Silvennoinen, Raimo},
   article_location = {Berlin Heidelberg},
   article_number = {2007},
   keywords = {amalgam electrodes; oligodeoxynucleotides detection},
   language = {eng},
   issn = {1432-1017},
   journal = {European Biophysics Journal},
   title = {Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface.},
   volume = {36},
   year = {2007}
}

TY  - JOUR
ID  - 749694
AU  - Vetterl, Vladimír - Vaněk, Jiří - Hasoň, Stanislav - Silvennoinen, Raimo
PY  - 2007
TI  - Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface.
JF  - European Biophysics Journal
VL  - 36
IS  - 2007
SP  - 204
EP  - 204
PB  - Springer-Verlag
SN  - 14321017
KW  - amalgam electrodes
KW  - oligodeoxynucleotides detection
N2  - The application of a gold amalgam-alloy electrode (AuAE) for a sensitive voltammetric detection of different oligodeoxynucleotides (ODNs) containing the purine units within the ODN-chains in the presence of copper ions is described. Our result shows that the sensitive detection of different ODNs can be also performed at the platinum amalgam-alloy electrode (PtAE) contrary to a low sensitivity at the silver amalgam-alloy electrode (AgAE).Electrochemical impedance spectroscopy (EIS), voltammetry, AFM, and DOE was used for the investigations of adsorption of oligonucleotides on the titanium surface and the effect of mechanical (polished, etched and/or gritted surface) and chemical modification of titanium surface was studied. From the EIS, voltammetric and optical measurements the conclusions of the effect of solvent and physical or chemical modification of titanium surface on the purine (AAG)12 and pyrimidine (TTC)12 oligonucleotide adsorption was delivered.
ER  -

VETTERL, Vladimír, Jiří VANĚK, Stanislav HASOŇ and Raimo SILVENNOINEN. Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface. \textit{European Biophysics Journal}. Berlin Heidelberg: Springer-Verlag, 2007, vol.~36, No~2007, p.~204. ISSN~1432-1017.

Detailed Information on Publication Record