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@article{750722,
author = {Řeháková, Zuzana and Šinkora, Jiří and Vlková, Marcela and Vokurková, Doris and Österreicher, Jan and Vávrová, Jana and Driak, Daniel},
article_number = {Jul 26},
keywords = {CD27; B cells; dosimetry},
language = {eng},
issn = {0862-8408},
journal = {Physiol Res},
title = {CD27(+) peripheral blood B-cells are a useful biodosimetric marker in vitro},
volume = {26},
year = {2007}
}
TY - JOUR
ID - 750722
AU - Řeháková, Zuzana - Šinkora, Jiří - Vlková, Marcela - Vokurková, Doris - Österreicher, Jan - Vávrová, Jana - Driak, Daniel
PY - 2007
TI - CD27(+) peripheral blood B-cells are a useful biodosimetric marker in vitro
JF - Physiol Res
VL - 26
IS - Jul 26
PB - Academi
SN - 08628408
KW - CD27
KW - B cells
KW - dosimetry
N2 - The CD8(+) natural killer (NK) subpopulation has recently been identified as a fast and reliable biodosimetric indicator within human peripheral blood mononuclear cells (PBMC) in vitro. In irradiated and subsequently cultivated PBMC, a decrease of the relative number of intact CD3-CD8(+) lymphocytes 16 and 48 hr after treatment has allowed for estimating the received dose in the range of 0 - 10 Gy and lethal/sublethal dose discrimination, respectively. Here we show that suitable biodosimeters can also be found in the peripheral blood B-cell compartment. Multiparameter flow cytometric analysis of irradiated and subsequently cultivated human PBMC revealed that both the CD27(+) and CD21(-) B-cell subpopulations can be used as biodosimeters and the CD19(+)CD27(+) lymphocytes have proved useful for retrospective determination of the received dose in the range of 0 - 6 Gy. In addition, several CD19(+) lymphocyte subsets characterized by co expression of CD21, CD27 and CD38 have been shown to bear biodosimetric potential, too. However, when important parameters like the original size within the CD19(+) compartment, its radiation-induced changes and data variation had been taken into account, the CD27(+) subpopulation proved superior to the other B-cell subpopulations and subsets. It appears that, in the dose range of 0 - 6 Gy, the relative decrease of CD27(+) B lymphocytes provides more sensitive and reliable data than that of CD8(+) NK-cells due mainly to lower data variation. In contrast to CD27(+) B cells, the proportions of CD27(+) subpopulations of T-cells were not affected by irradiation. We have also proposed a simple experimental protocol based on full blood cultivation and three-color CD27/CD3/CD19 immunophenotyping as a time-saving and inexpensive approach for practical biodosimetric evaluations on simple, three-to-four color flow cytometers.
ER -
ŘEHÁKOVÁ, Zuzana, Jiří ŠINKORA, Marcela VLKOVÁ, Doris VOKURKOVÁ, Jan ÖSTERREICHER, Jana VÁVROVÁ and Daniel DRIAK. CD27(+) peripheral blood B-cells are a useful biodosimetric marker in vitro. \textit{Physiol Res}. Academi, vol.~26, Jul 26. ISSN~0862-8408. 2007.
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