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@article{750722, author = {Řeháková, Zuzana and Šinkora, Jiří and Vlková, Marcela and Vokurková, Doris and Österreicher, Jan and Vávrová, Jana and Driak, Daniel}, article_number = {Jul 26}, keywords = {CD27; B cells; dosimetry}, language = {eng}, issn = {0862-8408}, journal = {Physiol Res}, title = {CD27(+) peripheral blood B-cells are a useful biodosimetric marker in vitro}, volume = {26}, year = {2007} }
TY - JOUR ID - 750722 AU - Řeháková, Zuzana - Šinkora, Jiří - Vlková, Marcela - Vokurková, Doris - Österreicher, Jan - Vávrová, Jana - Driak, Daniel PY - 2007 TI - CD27(+) peripheral blood B-cells are a useful biodosimetric marker in vitro JF - Physiol Res VL - 26 IS - Jul 26 PB - Academi SN - 08628408 KW - CD27 KW - B cells KW - dosimetry N2 - The CD8(+) natural killer (NK) subpopulation has recently been identified as a fast and reliable biodosimetric indicator within human peripheral blood mononuclear cells (PBMC) in vitro. In irradiated and subsequently cultivated PBMC, a decrease of the relative number of intact CD3-CD8(+) lymphocytes 16 and 48 hr after treatment has allowed for estimating the received dose in the range of 0 - 10 Gy and lethal/sublethal dose discrimination, respectively. Here we show that suitable biodosimeters can also be found in the peripheral blood B-cell compartment. Multiparameter flow cytometric analysis of irradiated and subsequently cultivated human PBMC revealed that both the CD27(+) and CD21(-) B-cell subpopulations can be used as biodosimeters and the CD19(+)CD27(+) lymphocytes have proved useful for retrospective determination of the received dose in the range of 0 - 6 Gy. In addition, several CD19(+) lymphocyte subsets characterized by co expression of CD21, CD27 and CD38 have been shown to bear biodosimetric potential, too. However, when important parameters like the original size within the CD19(+) compartment, its radiation-induced changes and data variation had been taken into account, the CD27(+) subpopulation proved superior to the other B-cell subpopulations and subsets. It appears that, in the dose range of 0 - 6 Gy, the relative decrease of CD27(+) B lymphocytes provides more sensitive and reliable data than that of CD8(+) NK-cells due mainly to lower data variation. In contrast to CD27(+) B cells, the proportions of CD27(+) subpopulations of T-cells were not affected by irradiation. We have also proposed a simple experimental protocol based on full blood cultivation and three-color CD27/CD3/CD19 immunophenotyping as a time-saving and inexpensive approach for practical biodosimetric evaluations on simple, three-to-four color flow cytometers. ER -
ŘEHÁKOVÁ, Zuzana, Jiří ŠINKORA, Marcela VLKOVÁ, Doris VOKURKOVÁ, Jan ÖSTERREICHER, Jana VÁVROVÁ and Daniel DRIAK. CD27(+) peripheral blood B-cells are a useful biodosimetric marker in vitro. \textit{Physiol Res}. Academi, 2007, vol.~26, Jul 26. ISSN~0862-8408.
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