Detailed Information on Publication Record
2007
Genome Differences between Treponema pallidum subsp. pallidum Strain Nichols and T. paraluiscuniculi Strain Cuniculi A
STROUHAL, Michal, David ŠMAJS, Petra MATĚJKOVÁ, Erica SODERGREN, Anita G. AMIN et. al.Basic information
Original name
Genome Differences between Treponema pallidum subsp. pallidum Strain Nichols and T. paraluiscuniculi Strain Cuniculi A
Name in Czech
Genomové rozdíly mezi Treponema pallidum subsp. pallidum Nichols a T. paraluiscuniculi Cuniculi A
Authors
STROUHAL, Michal (203 Czech Republic), David ŠMAJS (203 Czech Republic, guarantor), Petra MATĚJKOVÁ (203 Czech Republic), Erica SODERGREN (840 United States of America), Anita G. AMIN (840 United States of America), Jerrilyn K. HOWELL (840 United States of America), Steven J. NORRIS (840 United States of America) and George M. WEINSTOCK (840 United States of America)
Edition
INFECTION AND IMMUNITY, The American Society for Microbiology, 2007, 0019-9567
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.996
RIV identification code
RIV/00216224:14110/07:00019192
Organization unit
Faculty of Medicine
UT WoS
000251341500036
Keywords in English
Treponema pallidum; Treponema paraluiscuniculi; restriction analysis
Změněno: 25/6/2009 15:47, prof. MUDr. David Šmajs, Ph.D.
V originále
The genome of Treponema paraluiscuniculi strain Cuniculi A was compared to the genome of the syphilis spirochete Treponema pallidum subsp. pallidum strain Nichols using DNA microarray hybridization, whole-genome fingerprinting, and DNA sequencing. Using these approaches, deletions, insertions, and prominent sequence changes were found in 38 gene homologs and six intergenic regions of the Cuniculi A genome when it was compared to the genome of T. pallidum subsp. pallidum Nichols. Most of the observed differences were localized in tpr loci and the vicinity of these loci. In addition, 14 other genes were found to contain frameshift mutations resulting in major changes in protein sequences. Analysis of restriction target sites representing 0.34% of the total genome length and DNA sequencing of three PCR products (0.46% of the total genome length) amplified from Cuniculi A chromosomal regions and comparison to the Nichols genome revealed a sequence similarity of 98.6 to 99.3%. These results are consistent with a close genetic relationship among the T. pallidum strains and subspecies and a strong, but relatively divergent connection between the human and rabbit pathogens.
In Czech
Byly identifikovány genomové rozdíly mezi Treponema pallidum subsp. pallidum Nichols a T. paraluiscuniculi Cuniculi A. Tyto výsledky byly diskutovány v kontextu dalších dat.
Links
| GA310/07/0321, research and development project |
| ||
| MSM0021622415, plan (intention) |
| ||
| NR8967, research and development project |
|