Diagnosis of syphilis by polymerase chain reaction and molecular typing of Treponema pallidum.

ŠMAJS, David, Petra MATĚJKOVÁ, Vladana WOZNICOVÁ and Z. VALIŠOVÁ. Diagnosis of syphilis by polymerase chain reaction and molecular typing of Treponema pallidum. Reviews in Medical Microbiology. 2006, vol. 17, No 4, p. 93-100. ISSN 0954-139X.

Basic information

Original name

Diagnosis of syphilis by polymerase chain reaction and molecular typing of Treponema pallidum.

Name in Czech

Diagnostika syfilis pomocí PCR a molekulární typizace Treponema pallidum.

Authors

ŠMAJS, David (203 Czech Republic, guarantor), Petra MATĚJKOVÁ (203 Czech Republic), Vladana WOZNICOVÁ (203 Czech Republic) and Z. VALIŠOVÁ (203 Czech Republic)

Edition

Reviews in Medical Microbiology, 2006, 0954-139X

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.800

RIV identification code

RIV/00216224:14110/06:00024613

Organization unit

Faculty of Medicine

UT WoS

000247259900001

Keywords in English

Diagnosis of syphilis; molecular typing;Treponema pallidum.

Tags

Diagnosis of syphilis, molecular typing, Treponema pallidum.

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Abstract

V originále

Treponema pallidum subspecies pallidum is the causative agent of syphilis, a sexually transmitted disease that still represents a major world health problem. Serology, the standard laboratory method used for the diagnosis of syphilis, has several limitations that preclude reliable diagnosis of syphilis in some cases. Direct methods of syphilis diagnosis are suboptimal because of low sensitivity and low specificity (microscopy), or because of costly and delayed test results (rabbit infectivity test). Recent status of PCR-based syphilis diagnosis and molecular typing schemes are reviewed. PCR detection of Treponema pallidum in various clinical samples showed high sensitivity, specificity and potential for molecular typing. PCR diagnosis is therefore considered an important auxiliary laboratory test for syphilis. Typing of Treponema pallidum strains isolated from patients will stimulate epidemiological studies of syphilis and will allow discrimination between re-infection and reactivation of infection.

In Czech

Je popisována diagnostika syfilis pomocí PCR a molekulární typizace Treponema pallidum.

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Links

GA310/04/0021, research and development project	Name: Postgenomické studie syfilitické spirochety Treponema pallidum Investor: Czech Science Foundation, Postgenomic studies on Treponema pallidum, the syphilis spirochete
MSM0021622415, plan (intention)	Name: Molekulární podstata buněčných a tkáňových regulací Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
NR8967, research and development project	Name: Molekulární typizace kmenů a izolátů Treponema pallidum subsp. pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů Investor: Ministry of Health of the CR