Complete sequence of low-copy-number plasmid MccC7-H22 of
probiotic Escherichia coli H22 and the prevalence of mcc genes
among human E. coli.

J 2008

Complete sequence of low-copy-number plasmid MccC7-H22 of probiotic Escherichia coli H22 and the prevalence of mcc genes among human E. coli.

ŠMAJS, David, Michal STROUHAL, Petra MATĚJKOVÁ, Darina ČEJKOVÁ, Luciana CURSINO et. al.

Basic information

Original name

Complete sequence of low-copy-number plasmid MccC7-H22 of probiotic Escherichia coli H22 and the prevalence of mcc genes among human E. coli.

Name in Czech

Kompletní sekvence plasmidu MccC7-H22 z probiotického kmene Escherichia coli H22 a prevalence genů mcc mezi lidskými kmeny E. coli

Authors

ŠMAJS, David (203 Czech Republic, guarantor), Michal STROUHAL (203 Czech Republic), Petra MATĚJKOVÁ (203 Czech Republic), Darina ČEJKOVÁ (203 Czech Republic), Luciana CURSINO (76 Brazil), Edmar CHARTONE-SOUZA (76 Brazil), Jan ŠMARDA (203 Czech Republic) and Andrea M.A. NASCIMENTO (76 Brazil)

Edition

Plasmid, Thomson Scientific, 2008, 0147-619X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.255

RIV identification code

RIV/00216224:14110/08:00025713

Organization unit

Faculty of Medicine

UT WoS

000253074300001

Keywords in English

Escherichia coli; Microcin C7; Plasmid copy number; Complete sequence

Abstract

ORIG CZ

V originále

The complete sequence of the plasmid MccC7-H22 encoding microcin C7, isolated from probiotic E. coli H22, was determined and analyzed. DNA of pMccC7-H22 comprises 32,014 bp and contains 39 predicted ORFs. Two main gene clusters, i.e., genes involved in plasmid replication and maintenance and genes encoding microcin C7 synthesis, are separated by several ORFs homologous to ORFs present in IS (insertion sequence) elements and transposons. Additional 14 ORFs code for proteins with similarities to known proteins (4 ORFs) or for hypothetical proteins with unknown function (10 ORFs). The differences in G + C content of individual ORFs and gene clusters of pMccC7-H22 indicate a mosaic structure for the plasmid, resulting from recombination events.

In Czech

Byla určena kompletní sekvence plasmidu MccC7-H22 z probiotického kmene Escherichia coli H22 a tato sekvence byla anotována. Navíc byla vyšetřena také prevalence genů mcc mezi lidskými kmeny E. coli.

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

ŠMAJS, David, Michal STROUHAL, Petra MATĚJKOVÁ, Darina ČEJKOVÁ, Luciana CURSINO, Edmar CHARTONE-SOUZA, Jan ŠMARDA and Andrea M.A. NASCIMENTO. Complete sequence of low-copy-number plasmid MccC7-H22 of probiotic Escherichia coli H22 and the prevalence of mcc genes among human E. coli. Plasmid. Thomson Scientific, 2008, vol. 59, No 1, p. 1-10. ISSN 0147-619X.

@article{750899,
   author = {Šmajs, David and Strouhal, Michal and Matějková, Petra and Čejková, Darina and Cursino, Luciana and ChartoneandSouza, Edmar and Šmarda, Jan and Nascimento, Andrea M.A.},
   article_number = {1},
   keywords = {Escherichia coli; Microcin C7; Plasmid copy number; Complete sequence},
   language = {eng},
   issn = {0147-619X},
   journal = {Plasmid},
   title = {Complete sequence of low-copy-number plasmid MccC7-H22 of probiotic Escherichia coli H22 and the prevalence of mcc genes among human E. coli.},
   url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WPF-4PYJF2P-1&_user=10&_coverDate=01%2F31%2F2008&_rdoc=2&_fmt=full&_orig=browse&_srch=doc-info(%23toc%236989%232008%23999409998%23677858%23FLA%23display%23Volume)&_cdi=6989&_sort=d&_docanchor=&_c},
   volume = {59},
   year = {2008}
}

TY  - JOUR
ID  - 750899
AU  - Šmajs, David - Strouhal, Michal - Matějková, Petra - Čejková, Darina - Cursino, Luciana - Chartone-Souza, Edmar - Šmarda, Jan - Nascimento, Andrea M.A.
PY  - 2008
TI  - Complete sequence of low-copy-number plasmid MccC7-H22 of probiotic Escherichia coli H22 and the prevalence of mcc genes among human E. coli.
JF  - Plasmid
VL  - 59
IS  - 1
SP  - 1-10
EP  - 1-10
PB  - Thomson Scientific
SN  - 0147619X
KW  - Escherichia coli
KW  - Microcin C7
KW  - Plasmid copy number
KW  - Complete sequence
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WPF-4PYJF2P-1&_user=10&_coverDate=01%2F31%2F2008&_rdoc=2&_fmt=full&_orig=browse&_srch=doc-info(%23toc%236989%232008%23999409998%23677858%23FLA%23display%23Volume)&_cdi=6989&_sort=d&_docanchor=&_c
N2  - The complete sequence of the plasmid MccC7-H22 encoding microcin C7, isolated from probiotic E. coli H22, was determined and analyzed. DNA of pMccC7-H22 comprises 32,014 bp and contains 39 predicted ORFs. Two main gene clusters, i.e., genes involved in plasmid replication and maintenance and genes encoding microcin C7 synthesis, are separated by several ORFs homologous to ORFs present in IS (insertion sequence) elements and transposons. Additional 14 ORFs code for proteins with similarities to known proteins (4 ORFs) or for hypothetical proteins with unknown function (10 ORFs). The differences in G + C content of individual ORFs and gene clusters of pMccC7-H22 indicate a mosaic structure for the plasmid, resulting from recombination events.
ER  -

ŠMAJS, David, Michal STROUHAL, Petra MATĚJKOVÁ, Darina ČEJKOVÁ, Luciana CURSINO, Edmar CHARTONE-SOUZA, Jan ŠMARDA and Andrea M.A. NASCIMENTO. Complete sequence of low-copy-number plasmid MccC7-H22 of probiotic Escherichia coli H22 and the prevalence of mcc genes among human E. coli. \textit{Plasmid}. Thomson Scientific, 2008, vol.~59, No~1, p.~1-10. ISSN~0147-619X.

Detailed Information on Publication Record