| HORKA, Marie, O. KUBICEK, Filip RŮŽIČKA, Veronika HOLÁ, I. MALINOVSKA a Karel ŠLAIS. Capillary isoelectric focusing of native and inactivated microorganisms. Journal of Chromatography A. Amsterdam: Elsevier, 2007, roč. 1155, č. 2, s. 164-171, 171 s. ISSN 0021-9673. |
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@article{751881,
author = {Horka, Marie and Kubicek, O. and Růžička, Filip and Holá, Veronika and Malinovska, I. and Šlais, Karel},
article_location = {Amsterdam},
article_number = {2},
keywords = {Capillary isoelectric focusing; native microorganisms; inactivated microorganisms},
language = {eng},
issn = {0021-9673},
journal = {Journal of Chromatography A},
title = {Capillary isoelectric focusing of native and inactivated microorganisms},
volume = {1155},
year = {2007}
}
TY - JOUR
ID - 751881
AU - Horka, Marie - Kubicek, O. - Růžička, Filip - Holá, Veronika - Malinovska, I. - Šlais, Karel
PY - 2007
TI - Capillary isoelectric focusing of native and inactivated microorganisms
JF - Journal of Chromatography A
VL - 1155
IS - 2
SP - 164-171
EP - 164-171
PB - Elsevier
SN - 00219673
KW - Capillary isoelectric focusing
KW - native microorganisms
KW - inactivated microorganisms
N2 - The research of microorganisms includes the development of methods for the inactivation of viruses and other microbes. It also means to efficiently eliminate the infectivity of microorganisms without damage of their integrity and structure. According to the results of the last 5 years the capillary electromigration techniques appear to be very perspective for the comparison of the methods applicable for inactivation in the diagnostics and study of the pathogens. In this paper we suggest the capillary isoelectric focusing of the model microorganisms, Escherichia coli, Staphylococcus epidermidis, Candida albicans and bacteriophage PhiX 174, native or inactivated by different procedures. UV detection and fluorometric detection for the dynamically modified microbes by pyrenebutanoate on the basis of the non-ionogenic tenside were used here. Isoelectric points of native and/or dynamically modified microorganisms and other properties were compared with those obtained after microorganisms inactivation. The segmental injection of the sample pulse enabled the reproducible and efficient capillary isoelectric focusing in different pH gradients. The low-molecular-weight pI markers were used for tracing of the pH gradient.
ER -
HORKA, Marie, O. KUBICEK, Filip RŮŽIČKA, Veronika HOLÁ, I. MALINOVSKA a Karel ŠLAIS. Capillary isoelectric focusing of native and inactivated microorganisms. \textit{Journal of Chromatography A}. Amsterdam: Elsevier, 2007, roč.~1155, č.~2, s.~164-171, 171 s. ISSN~0021-9673.
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