| MICHÁLEK, Jaroslav, Ivo KOCÁK, Vuk FAIT, Jan ŽALOUDÍK and Roman HÁJEK. Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma. Journal of immunology. Bethesda: American association of immunologists, 2007, vol. 2007, No 1, p. 178-189, 11 pp. ISSN 0022-1767. |
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@article{752159,
author = {Michálek, Jaroslav and Kocák, Ivo and Fait, Vuk and Žaloudík, Jan and Hájek, Roman},
article_location = {Bethesda},
article_number = {1},
keywords = {metastatic melanoma; T cell},
language = {eng},
issn = {0022-1767},
journal = {Journal of immunology},
title = {Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma.},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17513726?ordinalpos=4&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum},
volume = {2007},
year = {2007}
}
TY - JOUR
ID - 752159
AU - Michálek, Jaroslav - Kocák, Ivo - Fait, Vuk - Žaloudík, Jan - Hájek, Roman
PY - 2007
TI - Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma.
JF - Journal of immunology
VL - 2007
IS - 1
SP - 178-189
EP - 178-189
PB - American association of immunologists
SN - 00221767
KW - metastatic melanoma
KW - T cell
UR - http://www.ncbi.nlm.nih.gov/pubmed/17513726?ordinalpos=4&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum
N2 - We investigated the presence of individual melanoma-specific T cell clones in patients with metastatic melanoma. Ten patients were examined for the presence of melanoma-reactive T cells using dendritic cells loaded with autologous tumor cells. Their specificity was tested using nonradioactive cytotoxicity test. Individual immumodominant T cell clones were identified by the clonotypic assay that combines in vitro cell culture, immunomagnetic sorting of activated IFN-gamma(+) T cells, TCR beta locus-anchored RT-PCR, and clonotypic quantitative PCR. All patients had detectable melanoma-reactive T cells in vitro. Expanded melanoma-reactive T cells demonstrated specific cytotoxic effect against autologous tumor cells in vitro. Three patients experienced objective responses, and their clinical responses were closely associated with the in vivo expansion and long-term persistence of individual CD8(+) T cell clones with frequencies of 10(-6) to 10(-3) of all circulating CD8+ T cells. Five patients with progressive disease experienced no or temporary presence of circulating melanoma-reactive T cell clones. Thus, circulating immunodominant CD8(+) T cell clones closely correlate with clinical outcome inpatients with metastatic melanoma.
ER -
MICHÁLEK, Jaroslav, Ivo KOCÁK, Vuk FAIT, Jan ŽALOUDÍK and Roman HÁJEK. Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma. \textit{Journal of immunology}. Bethesda: American association of immunologists, 2007, vol.~2007, No~1, p.~178-189, 11 pp. ISSN~0022-1767.
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