Detection and long-term in vivo monitoring of individual
tumor-specific T cell clones in patients with metastatic
melanoma.

J 2007

Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma.

MICHÁLEK, Jaroslav, Ivo KOCÁK, Vuk FAIT, Jan ŽALOUDÍK, Roman HÁJEK et. al.

Basic information

Original name

Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma.

Name in Czech

Detekce a dlouhodobé in vivo monitorování individuálních nádorově specifických klonů T lymfocytů u pacientů s metastázujícím melanomem.

Authors

MICHÁLEK, Jaroslav (203 Czech Republic, guarantor), Ivo KOCÁK (203 Czech Republic), Vuk FAIT (203 Czech Republic), Jan ŽALOUDÍK (203 Czech Republic) and Roman HÁJEK (203 Czech Republic)

Edition

Journal of immunology, Bethesda, American association of immunologists, 2007, 0022-1767

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.068

RIV identification code

RIV/00216224:14110/07:00033038

Organization unit

Faculty of Medicine

UT WoS

000246896300018

Keywords in English

metastatic melanoma; T cell

Abstract

ORIG CZ

V originále

We investigated the presence of individual melanoma-specific T cell clones in patients with metastatic melanoma. Ten patients were examined for the presence of melanoma-reactive T cells using dendritic cells loaded with autologous tumor cells. Their specificity was tested using nonradioactive cytotoxicity test. Individual immumodominant T cell clones were identified by the clonotypic assay that combines in vitro cell culture, immunomagnetic sorting of activated IFN-gamma(+) T cells, TCR beta locus-anchored RT-PCR, and clonotypic quantitative PCR. All patients had detectable melanoma-reactive T cells in vitro. Expanded melanoma-reactive T cells demonstrated specific cytotoxic effect against autologous tumor cells in vitro. Three patients experienced objective responses, and their clinical responses were closely associated with the in vivo expansion and long-term persistence of individual CD8(+) T cell clones with frequencies of 10(-6) to 10(-3) of all circulating CD8+ T cells. Five patients with progressive disease experienced no or temporary presence of circulating melanoma-reactive T cell clones. Thus, circulating immunodominant CD8(+) T cell clones closely correlate with clinical outcome inpatients with metastatic melanoma.

In Czech

Detekce a dlouhodobé in vivo monitorování individuálních nádorově specifických klonů T lymfocytů u pacientů s metastázujícím melanomem.

Citovat

MICHÁLEK, Jaroslav, Ivo KOCÁK, Vuk FAIT, Jan ŽALOUDÍK and Roman HÁJEK. Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma. Journal of immunology. Bethesda: American association of immunologists, 2007, vol. 2007, No 1, p. 178-189, 11 pp. ISSN 0022-1767.

@article{752159,
   author = {Michálek, Jaroslav and Kocák, Ivo and Fait, Vuk and Žaloudík, Jan and Hájek, Roman},
   article_location = {Bethesda},
   article_number = {1},
   keywords = {metastatic melanoma; T cell},
   language = {eng},
   issn = {0022-1767},
   journal = {Journal of immunology},
   title = {Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma.},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/17513726?ordinalpos=4&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum},
   volume = {2007},
   year = {2007}
}

TY  - JOUR
ID  - 752159
AU  - Michálek, Jaroslav - Kocák, Ivo - Fait, Vuk - Žaloudík, Jan - Hájek, Roman
PY  - 2007
TI  - Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma.
JF  - Journal of immunology
VL  - 2007
IS  - 1
SP  - 178-189
EP  - 178-189
PB  - American association of immunologists
SN  - 00221767
KW  - metastatic melanoma
KW  - T cell
UR  - http://www.ncbi.nlm.nih.gov/pubmed/17513726?ordinalpos=4&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum
N2  - We investigated the presence of individual melanoma-specific T cell clones in patients with metastatic melanoma. Ten patients were examined for the presence of melanoma-reactive T cells using dendritic cells loaded with autologous tumor cells. Their specificity was tested using nonradioactive cytotoxicity test. Individual immumodominant T cell clones were identified by the clonotypic assay that combines in vitro cell culture, immunomagnetic sorting of activated IFN-gamma(+) T cells, TCR beta locus-anchored RT-PCR, and clonotypic quantitative PCR. All patients had detectable melanoma-reactive T cells in vitro. Expanded melanoma-reactive T cells demonstrated specific cytotoxic effect against autologous tumor cells in vitro. Three patients experienced objective responses, and their clinical responses were closely associated with the in vivo expansion and long-term persistence of individual CD8(+) T cell clones with frequencies of 10(-6) to 10(-3) of all circulating CD8+ T cells. Five patients with progressive disease experienced no or temporary presence of circulating melanoma-reactive T cell clones. Thus, circulating immunodominant CD8(+) T cell clones closely correlate with clinical outcome inpatients with metastatic melanoma.
ER  -

MICHÁLEK, Jaroslav, Ivo KOCÁK, Vuk FAIT, Jan ŽALOUDÍK and Roman HÁJEK. Detection and long-term in vivo monitoring of individual tumor-specific T cell clones in patients with metastatic melanoma. \textit{Journal of immunology}. Bethesda: American association of immunologists, 2007, vol.~2007, No~1, p.~178-189, 11 pp. ISSN~0022-1767.

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