ŠKAREK, Michal, Jaroslav JANOŠEK, Pavel ČUPR, Jiří KOHOUTEK, A. NOVOTNÁ - RYCHETSKÁ and Ivan HOLOUBEK. Evaluation of genotoxic and non-genotoxic effects of organic air pollution using in vitro bioassays. Environment International. 2007, 33/7/2007, 33/7/2007, p. 859-866. ISSN 0160-4120.
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Basic information
Original name Evaluation of genotoxic and non-genotoxic effects of organic air pollution using in vitro bioassays
Name in Czech Hodnocení genotoxických a negenotoxických efektů organických polutantů volného ovzduší s využitím in vitro testů
Authors ŠKAREK, Michal (203 Czech Republic), Jaroslav JANOŠEK (203 Czech Republic), Pavel ČUPR (203 Czech Republic, guarantor), Jiří KOHOUTEK (203 Czech Republic), A. NOVOTNÁ - RYCHETSKÁ (203 Czech Republic) and Ivan HOLOUBEK (203 Czech Republic).
Edition Environment International, 2007, 0160-4120.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10511 Environmental sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.797
RIV identification code RIV/00216224:14310/07:00023700
Organization unit Faculty of Science
UT WoS 000249677300001
Keywords in English Air pollution; PAHs; Genotoxicity; Dioxin toxicity; Bioassay
Tags air pollution, bioassay, Dioxin toxicity, genotoxicity, PaHs
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Pavel Čupr, Ph.D., učo 16144. Changed: 30/1/2008 09:45.
Abstract
In this study, organic extracts of total suspended particles (TSP) and the particulate matter (PM) with the size below 2.5 um (PM2.5) combined with organic extracts of the gas phase (GP) collected at two urban and two background localities were analyzed with a bacterial genotoxicity test, SOS chromotest, and an in vitro test for the dioxin toxicity determination, using a modified cell-line of rat hepatoma H4IIE.luc. In addition, the samples of TSP and GP were analyzed for PAHs contents. The PAHs concentrations and both of the toxic activities at the urban localities were much higher than ones at the background localities. Predominantly, traffic was a source of the urban air pollution there which was also confirmed by the evaluation of portions of certain PAHs (BaP/BPE, PYR/BaP) at the localities. On the other hand, the background localities were apparently affected by a long-distance transport of the pollutants from urban and industrial centers. The results of the bioassays indicated potential health risks for the population exposed to the organic air pollutants, especially at the urban localities. Based on the collected samples, distribution of the organic pollutants with the toxic effects in the air was evaluated. The significant portion of the direct genotoxins was bound to the particles larger than 2.5 um. On the contrary, the indirect genotoxins were bound predominantly to the particles with the size below 2.5 um. However, in the urban air they may be also bound to the larger particles, as well. While the direct genotoxicity may be related with the presence of PAH-derivatives as well as some polar organic pollutants, the indirect genotoxicity is related with the detected carcinogenic PAHs. But besides the above specified pollutants it is also necessary to consider the presence of other toxic components of the complex organic air pollution mixture that may also show potential health risks. This study demonstrates application of the combination of the screening bioassays for the evaluation of organic air pollution and identification of its health risks.
Abstract (in Czech)
In this study, organic extracts of total suspended particles (TSP) and the particulate matter (PM) with the size below 2.5 um (PM2.5) combined with organic extracts of the gas phase (GP) collected at two urban and two background localities were analyzed with a bacterial genotoxicity test, SOS chromotest, and an in vitro test for the dioxin toxicity determination, using a modified cell-line of rat hepatoma H4IIE.luc. In addition, the samples of TSP and GP were analyzed for PAHs contents. The PAHs concentrations and both of the toxic activities at the urban localities were much higher than ones at the background localities. Predominantly, traffic was a source of the urban air pollution there which was also confirmed by the evaluation of portions of certain PAHs (BaP/BPE, PYR/BaP) at the localities. On the other hand, the background localities were apparently affected by a long-distance transport of the pollutants from urban and industrial centers. The results of the bioassays indicated potential health risks for the population exposed to the organic air pollutants, especially at the urban localities. Based on the collected samples, distribution of the organic pollutants with the toxic effects in the air was evaluated. The significant portion of the direct genotoxins was bound to the particles larger than 2.5 um. On the contrary, the indirect genotoxins were bound predominantly to the particles with the size below 2.5 um. However, in the urban air they may be also bound to the larger particles, as well. While the direct genotoxicity may be related with the presence of PAH-derivatives as well as some polar organic pollutants, the indirect genotoxicity is related with the detected carcinogenic PAHs. But besides the above specified pollutants it is also necessary to consider the presence of other toxic components of the complex organic air pollution mixture that may also show potential health risks. This study demonstrates application of the combination of the screening bioassays for the evaluation of organic air pollution and identification of its health risks.
Links
MSM0021622412, plan (intention)Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
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