Determination of D- and L-amino acids produced by cyanobacteria
using gas chromatography on Chirasil-Val after derivatization
with pentafluoropropyl chloroformate

J 2007

Determination of D- and L-amino acids produced by cyanobacteria using gas chromatography on Chirasil-Val after derivatization with pentafluoropropyl chloroformate

ZAHRADNÍČKOVÁ, Helena and Ivan HOLOUBEK

Basic information

Original name

Determination of D- and L-amino acids produced by cyanobacteria using gas chromatography on Chirasil-Val after derivatization with pentafluoropropyl chloroformate

Name in Czech

Determination of D- and L-amino acids produced by cyanobacteria using gas chromatography on Chirasil-Val after derivatization with pentafluoropropyl chloroformate

Authors

ZAHRADNÍČKOVÁ, Helena (203 Czech Republic, guarantor, belonging to the institution) and Ivan HOLOUBEK (203 Czech Republic, belonging to the institution)

Edition

Analytical and Bioanalytical Chemistry, Heidelberg, Springer-Verlag GmbH, 2007, 1618-2642

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.867

RIV identification code

RIV/00216224:14310/07:00041790

Organization unit

Faculty of Science

UT WoS

000248373300031

Keywords in English

Amino acids; cyanobacteria; gas chromatography

Abstract

ORIG CZ

V originále

A rapid and simple method was developed for the determination of free amino acids (AAs) released from cyanobacteria. The procedure involves trapping of AAs from the centrifuged cyanobacterial culture fluid on a cation-exchange resin, their release together with the resin by a direct treating with the reaction medium, followed by immediate derivatization with a respective chloroformate.

In Czech

Vyvinuta byla rychlá a jednoduchá metoda determinace volných aminokyslin uvolnovaných ze sinic. Metoda zahrnuje zachyceni AA z centrifugované kultury na kation-výměnném resinu a uvolnění do reakčního media a derivatizaci.

Links

MSM0021622412, plan (intention)

Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

Detailed Information on Publication Record