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@proceedings{753244,
author = {Kozubek, Michal and Matula, Petr and Matula, Pavel and Vařecha, Miroslav and Amrichová, Jana and Ondřej, Vladan and Lukášová, Emilie and Kozubek, Stanislav},
booktitle = {Focus on Microscopy: Program and Abstract Book},
keywords = {confocal microscopy; image processing},
language = {eng},
title = {Automated confocal in vivo microscopy based on spinning disks},
year = {2005}
}
TY - CONF
ID - 753244
AU - Kozubek, Michal - Matula, Petr - Matula, Pavel - Vařecha, Miroslav - Amrichová, Jana - Ondřej, Vladan - Lukášová, Emilie - Kozubek, Stanislav
PY - 2005
TI - Automated confocal in vivo microscopy based on spinning disks
KW - confocal microscopy
KW - image processing
N2 - The successful development of non-invasive quantitative visualization techniques for live cell imaging have led to the development of suitable hardware and software for the acquisition and processing of multidimensional image data. Confocal spinning disk systems (based either on a classical Nipkow disk or on the microlens principle) are especially suitable for in vivo imaging thanks to high acquisition speed (parallel imaging of thousands of points), high quality image detection (up to 90% quantum efficiency at negligible noise levels with state-of-the-art cameras), low photobleaching and low phototoxicity. Lately, we have been working on the optimization and automation of image acquisition and processing for this type of microscopy. Special attention has been paid to ultra-fast image acquisition mode. This poster presents both hardware as well as software approach used in our laboratory. It also shows examples of biological tasks that are being solved using the automated confocal in vivo microscopy systems.
ER -
KOZUBEK, Michal, Petr MATULA, Pavel MATULA, Miroslav VAŘECHA, Jana AMRICHOVÁ, Vladan ONDŘEJ, Emilie LUKÁŠOVÁ and Stanislav KOZUBEK. Automated confocal in vivo microscopy based on spinning disks. In \textit{Focus on Microscopy: Program and Abstract Book}. 2005.
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