ŠVÁBENSKÝ, Radim, Kamila KOČÍ and Zdeněk ŠIMEK. The study of properties of HPLC determination of polycyclic aromatic nitrogen heterocycles. International Journal of Environmental Analytical Chemistry. 2007, vol. 87, No 5, p. 337-349. ISSN 1029-0397.
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Basic information
Original name The study of properties of HPLC determination of polycyclic aromatic nitrogen heterocycles
Name in Czech Studium podmínek HPLC stanovení polycyclických aromatických dusíkatých heterocyklů
Authors ŠVÁBENSKÝ, Radim (203 Czech Republic), Kamila KOČÍ (203 Czech Republic) and Zdeněk ŠIMEK (203 Czech Republic, guarantor).
Edition International Journal of Environmental Analytical Chemistry, 2007, 1029-0397.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.026
RIV identification code RIV/00216224:14310/07:00023783
Organization unit Faculty of Science
UT WoS 000244920500003
Keywords in English polycyclic aromatic nitrogen heterocycles; azaarenes; PANHs; PAHs;
Tags azaarenes, PaHs, PANHs, Polycyclic Aromatic Nitrogen Heterocycles
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Zdeněk Šimek, CSc., učo 37019. Changed: 31/1/2008 15:55.
Abstract
The study of the separation of polycyclic aromatic nitrogen heterocycles (PANHs) by reversephase liquid chromatography with an octadecyl stationary phase is presented. The retention behaviour of a mixture of PANHs was studied under different chromatographic conditions. A mixture of phosphate buffer/acetonitrile was used as mobile phase in isocratic and gradient modes. The effect of different pH mobile phase in the range from 2.5 to 6.5 has been investigated to describe retention changes of PANHs as a function of their acid/base properties. Different concentrations of phosphate buffer as a component of the mobile phase were used to study the effect of ionic strength. Very good RP-HPLC separation of 24 PANHs and 16 EPA polycyclic aromatic hydrocarbons (PAHs) was obtained without a pre-separation step in a test mixture and the extract of a real soil sample. Limits of detection of PANHs obtained by two detection techniques, ultraviolet-diode array detection (UV-DAD) and fluorescence detector (FD), are compared. The proposed method is tested with a real soil sample.
Abstract (in Czech)
The study of the separation of polycyclic aromatic nitrogen heterocycles (PANHs) by reversephase liquid chromatography with an octadecyl stationary phase is presented. The retention behaviour of a mixture of PANHs was studied under different chromatographic conditions. A mixture of phosphate buffer/acetonitrile was used as mobile phase in isocratic and gradient modes. The effect of different pH mobile phase in the range from 2.5 to 6.5 has been investigated to describe retention changes of PANHs as a function of their acid/base properties. Different concentrations of phosphate buffer as a component of the mobile phase were used to study the effect of ionic strength. Very good RP-HPLC separation of 24 PANHs and 16 EPA polycyclic aromatic hydrocarbons (PAHs) was obtained without a pre-separation step in a test mixture and the extract of a real soil sample. Limits of detection of PANHs obtained by two detection techniques, ultraviolet-diode array detection (UV-DAD) and fluorescence detector (FD), are compared. The proposed method is tested with a real soil sample.
Links
MSM0021622412, plan (intention)Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
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