Detailed Information on Publication Record
2007
TSPY gene copy number as a potential new risk factor for male infertility
VODICKA, R., R. VRTEL, Ladislav DUŠEK, A.R. SINGH, K. KRIZOVA et. al.Basic information
Original name
TSPY gene copy number as a potential new risk factor for male infertility
Name in Czech
TSPY gene copy number as a potential new risk factor for male infertility
Authors
VODICKA, R. (203 Czech Republic), R. VRTEL (203 Czech Republic), Ladislav DUŠEK (203 Czech Republic, guarantor), A.R. SINGH (203 Czech Republic), K. KRIZOVA (203 Czech Republic), V. SVACINOVA (203 Czech Republic), V. HORINOVA (203 Czech Republic), J. DOSTAL (203 Czech Republic), I. OBORNA (203 Czech Republic), J. BREZINOVA (203 Czech Republic), A. SOBEK (203 Czech Republic) and J. SANTAVY (203 Czech Republic)
Edition
Reproductive Biomedicine Online, 2007, 1472-6491
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30214 Obstetrics and gynaecology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.840
RIV identification code
RIV/00216224:14110/07:00040020
Organization unit
Faculty of Medicine
UT WoS
000246445000009
Keywords in English
capillary electrophoresis; male infertility; multicopy gene; quantitative fluorescence PCR; TSPY gene; Y chromosome
Tags
Změněno: 1/4/2010 08:44, prof. RNDr. Ladislav Dušek, Ph.D.
V originále
The human TSPY (testis-specific protein, Y-linked) gene family (30-60 copies) is situated in the MSY (male-specific) region of the Y chromosome. Testis-specific expression indicates that the gene plays a role in spermatogenesis. Refined quantitative fluorescence PCR (polymerase chain reaction) was applied to evaluate the relative number of TSPY copies compared with AMELY/X (amelogenin gene, Y-linked) genes in 84 stratified infertile men and in 40 controls. A significantly higher number of TSPY copies was found in infertile men compared with the controls (P = 0.002). The diagnostic discrimination potential of the relative number of TSPY copies was evaluated by receiver operating characteristic curve analysis. TSPY/AMELY was unambiguously found to be powerful in the diagnostic separation of both the control samples and the infertile men, reaching a good level of specificity (0.642) and sensitivity (0.732) at a cut-off point of 0.46. The findings were supported by independently repeated studies of randomly selected positive samples and controls. Evaluation of the TSPY copy number offers a completely new diagnostic approach in relation to the genetic cause of male infertility. The possible effect of the copy number of TSPY genes on spermatogenesis may explain indiscrete pathological alterations of spermatid quality and quantity.
In Czech
The human TSPY (testis-specific protein, Y-linked) gene family (30-60 copies) is situated in the MSY (male-specific) region of the Y chromosome. Testis-specific expression indicates that the gene plays a role in spermatogenesis. Refined quantitative fluorescence PCR (polymerase chain reaction) was applied to evaluate the relative number of TSPY copies compared with AMELY/X (amelogenin gene, Y-linked) genes in 84 stratified infertile men and in 40 controls. A significantly higher number of TSPY copies was found in infertile men compared with the controls (P = 0.002). The diagnostic discrimination potential of the relative number of TSPY copies was evaluated by receiver operating characteristic curve analysis. TSPY/AMELY was unambiguously found to be powerful in the diagnostic separation of both the control samples and the infertile men, reaching a good level of specificity (0.642) and sensitivity (0.732) at a cut-off point of 0.46. The findings were supported by independently repeated studies of randomly selected positive samples and controls. Evaluation of the TSPY copy number offers a completely new diagnostic approach in relation to the genetic cause of male infertility. The possible effect of the copy number of TSPY genes on spermatogenesis may explain indiscrete pathological alterations of spermatid quality and quantity.
Links
| NR8442, research and development project |
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