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@inproceedings{755039,
author = {Koča, Jaroslav},
address = {Brno},
booktitle = {CECE 2007},
keywords = {protein enginnering lectin in silico},
language = {eng},
location = {Brno},
pages = {22-22},
publisher = {JAC},
title = {In silico and In vitro Protein Engineering as a Bioanalytical Tool},
year = {2007}
}
TY - JOUR
ID - 755039
AU - Koča, Jaroslav
PY - 2007
TI - In silico and In vitro Protein Engineering as a Bioanalytical Tool
PB - JAC
CY - Brno
KW - protein enginnering lectin in silico
N2 - Protein engineering is a procedure of developing useful or valuable proteins by mutations of residues. It is usually performed in vitro, when the original DNA is mutated, incorporated into host cells (usually E. coli) and the resulting protein with mutated residues is expressed. It can also be performed in silico, when mutations are performed on a model in a computer. Our effort is to connect both approaches to increase efficiency of the procedure. In our case, the engineered molecules are called lectins, nonenzymatic proteins that recognize carbohydrates. The lecture will be mainly focused on in silico part of the procedure, which is performed by the program TRITON developed in our laboratory. During this procedure, mutants are automatically generated, refined and their affinity and specificity is predicted. The method is then complemented by in vitro approaches that finish with thermodynamic and kinetic measurements.
ER -
KOČA, Jaroslav. In silico and In vitro Protein Engineering as a Bioanalytical Tool. In \textit{CECE 2007}. Brno: JAC, 2007, p.~22-22.
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