Detailed Information on Publication Record
2008
Determination of Asymmetric Dimethylarginine in Human Plasma - Comparison HPLC Methods with Fluorescent or Coulometric Detection with ELISA Method
KLEPÁRNÍK, Martin and Josef TOMANDLBasic information
Original name
Determination of Asymmetric Dimethylarginine in Human Plasma - Comparison HPLC Methods with Fluorescent or Coulometric Detection with ELISA Method
Name in Czech
STANOVENÍ ASYMETRICKÉHO DIMETHYLARGININU V LIDSKÉ PLAZMĚ - SROVNÁNÍ HPLC METOD S FLUORESCENČNÍ A COULOMETRICKOU DETEKCÍ S ELISA METODOU
Authors
KLEPÁRNÍK, Martin and Josef TOMANDL
Edition
Brno, Sborník příspěvků, p. 74-75, 117 pp. 2008
Publisher
Masarykova univerzita
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Organization unit
Faculty of Medicine
ISBN
978-80-210-4526-2
Keywords in English
ADMA; HPLC; ELISA
Změněno: 3/12/2008 20:31, doc. RNDr. Josef Tomandl, Ph.D.
V originále
Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. The increase of concentration leads to increased blood pressure and reduced blood flow. Elevated plasma ADMA is an independent risk factor for endotelial dysfunction and has been associated with e.g. hypertension, diabetes mellitus, hypercholesterolemia, renal failure and atherosclerosis. In our correlation study we used EDTA-plasma samples from patients 24h after acute myocardial infarction and ADMA concentrations, obtained by HPLC with fluorescent or coulometric detection and ELISA method, were compared and among methods was poor correlation. Detection limits of HPLC methods was approximately 5times lower than in ELISA method and 50times lower than in plasma of healthy people.
In Czech
Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. The increase of concentration leads to increased blood pressure and reduced blood flow. Elevated plasma ADMA is an independent risk factor for endotelial dysfunction and has been associated with e.g. hypertension, diabetes mellitus, hypercholesterolemia, renal failure and atherosclerosis. In our correlation study we used EDTA-plasma samples from patients 24h after acute myocardial infarction and ADMA concentrations, obtained by HPLC with fluorescent or coulometric detection and ELISA method, were compared and among methods was poor correlation. Detection limits of HPLC methods was approximately 5times lower than in ELISA method and 50times lower than in plasma of healthy people.
Links
| LC06023, research and development project |
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| MSM0021622402, plan (intention) |
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