Detailed Information on Publication Record
2008
Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as Bioindicators of Environmental Pollution
KŘÍŽKOVÁ, Soňa, Pavel RYANT, Olga KRYŠTOFOVÁ, Vojtěch ADAM, Michaela GALIOVÁ et. al.Basic information
Original name
Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as Bioindicators of Environmental Pollution
Name in Czech
Multi-instrumentální analýza tkání slunečníce dopoované stříbrnými ionty - Rostliny jako indikátory zněčištění životního prostředí
Authors
KŘÍŽKOVÁ, Soňa (203 Czech Republic), Pavel RYANT (203 Czech Republic), Olga KRYŠTOFOVÁ (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Michaela GALIOVÁ (203 Czech Republic), Miroslava BEKLOVÁ (203 Czech Republic), Petr BABULA (203 Czech Republic), Jozef KAISER (203 Czech Republic), Karel NOVOTNÝ (203 Czech Republic), Jan NOVOTNÝ (203 Czech Republic), Miroslav LIŠKA (203 Czech Republic), Radomír MALINA (203 Czech Republic), Josef ZEHNÁLEK (203 Czech Republic), Jaromír HUBÁLEK (203 Czech Republic), Ladislav HAVEL (203 Czech Republic) and René KIZEK (203 Czech Republic, guarantor)
Edition
Sensors, 2008, 1424-8220
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.870
RIV identification code
RIV/00216224:14310/08:00025821
Organization unit
Faculty of Science
UT WoS
000252628400025
Keywords in English
silver; heavy metals; plant biosensor; sensors; biochemical marker
Tags
International impact
Změněno: 8/7/2009 17:57, prof. RNDr. Vojtěch Adam, Ph.D.
V originále
The aim of this work is to investigate sunflower plants response on stress induced by silver(I) ions. The sunflower plants were exposed to silver(I) ions (0, 0.1, 0.5, and 1 mM) for 96 h. Primarily we aimed our attention to observation of basic physiological parameters. We found that the treated plants embodied growth depression, coloured changes and lack root hairs. Using of autofluorescence of anatomical structures, such as lignified cell walls, it was possible to determine the changes of important shoot and root structures, mainly vascular bungles and development of secondary thickening. The differences in vascular bundles organisation, parenchymatic pith development in the root centre and the reduction of phloem part of vascular bundles were well observable. Moreover with increasing silver(I) ions concentration the vitality of rhizodermal cells declined; rhizodermal cells early necrosed and were replaced by the cells of exodermis. Further we employed laser induced breakdown spectroscopy for determination of spatial distribution of silver(I) ions in tissues of the treated plants. The Ag is accumulated mainly in near-root part of the sample. Moreover basic biochemical indicators of environmental stress were investigated. The total content of proteins expressively decreased with increasing silver(I) ions dose and the time of the treatment. As we compare the results obtained by protein analysis-the total protein contents in shoot as well as root parts-we can assume on the transport of the proteins from the roots to shoots. This phenomenon can be related with the cascade of processes connecting with photosynthesis. The second biochemical parameter, which we investigated, was urease activity. If we compared the activity in treated plants with control, we found out that presence of silver(I) ions markedly enhanced the activity of urease at all applied doses of this toxic metal. Finally we studied the effect of silver(I) ions on activity of urease in in vitro conditions.
In Czech
Cílem práce je studim reakcí rostlin slunečnice na stres zpusobený stříbrnými ionty. Rostliny slunečnice byly vystaveny působení stříbrných iontů (o koncentraci 0, 0.1, 0.5 a 1 mM) po dobu 96 hodin. V první řadě byla venována pozornost základním fyziologickým parametrům. Byl zjištěn snížený růst dopovaných rostlin, změna barvy a zmenšení kořenového systému. S použitím autofluorescence anatomických struktur byly možno určit změny na výhoncích a kořenech. Dále byla použita spektrometrie laserem buzeného plazmatu pro určení prostorové distribuce stříbrných iontů v tkáních. Bylo zjištěno že se střibro akumuluje v tkáních blízko kořenů.
Links
| MSM0021622411, plan (intention) |
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