Characterization of Lactococcus lactis subsp. lactis isolated
from surface waters

J 2008

Characterization of Lactococcus lactis subsp. lactis isolated from surface waters

ŠVEC, Pavel and Ivo SEDLÁČEK

Basic information

Original name

Characterization of Lactococcus lactis subsp. lactis isolated from surface waters

Name in Czech

Charakteristika Lactococcus lactis subsp. lactis izolovaných z povrchových vod

Authors

ŠVEC, Pavel (203 Czech Republic, guarantor) and Ivo SEDLÁČEK (203 Czech Republic)

Edition

Folia Microbiologica, PRINCO International, Praha, Springer, 2008, 0015-5632

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.172

RIV identification code

RIV/00216224:14310/08:00025866

Organization unit

Faculty of Science

UT WoS

000254752900007

Keywords in English

Lactococcus lactis subsp. lactis; water; taxonomy; identification

Abstract

ORIG CZ

V originále

A group of nine presumptive enterococci was isolated on enterococcal selective media Slanetz-Bartley agar and/or Kanamycin esculin azide agar during a screening of Enterococcus spp. in surface waters. All strains formed a homogeneous cluster separated from all enterococcal species using the rep-PCR fingerprinting with the (GTG)5 primer, but they matched fingerprints revealed by Lactococcus lactis subsp. lactis representatives. Further identification using extensive biotyping and automated ribotyping with EcoRI (RiboPrinter microbial characterization system) confirmed all strains as L. lactis subsp. lactis in full correspondence with the (GTG)5-PCR. We demonstrated that L. lactis subsp. lactis strains occur in different surface waters and can be confused with enterococci due to their positive growth on selective enterococcal media as well as positive results in tests commonly used for identification of the genus Enterococcus (esculin hydrolysis, acetoin and pyrrolidonyl arylamidase production, growth at 10 C and in 6.5 % NaCl). The (GTG)5-PCR fingerprinting revealed as a reliable and fast method for the identification of L. lactis subsp. lactis while automated ribotyping with EcoRI proved to be a good tool for intrasubspecies typing purposes.

In Czech

Charakteristika a identifikace kmenů Lactococcus lactis subsp. lactis izolovaných z povrchových vod metodou (GTG)5-PCR fingerprinting.

Links

MSM0021622416, plan (intention)

Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time

Citovat

ŠVEC, Pavel and Ivo SEDLÁČEK. Characterization of Lactococcus lactis subsp. lactis isolated from surface waters. Folia Microbiologica. PRINCO International, Praha: Springer, 2008, vol. 53, No 1, p. 53-56. ISSN 0015-5632.

@article{763561,
   author = {Švec, Pavel and Sedláček, Ivo},
   article_location = {PRINCO International, Praha},
   article_number = {1},
   keywords = {Lactococcus lactis subsp. lactis; water; taxonomy; identification},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {Characterization of Lactococcus lactis subsp. lactis isolated from surface waters},
   url = {http://www.biomed.cas.cz/mbu/folia/contents/2008.html},
   volume = {53},
   year = {2008}
}

TY  - JOUR
ID  - 763561
AU  - Švec, Pavel - Sedláček, Ivo
PY  - 2008
TI  - Characterization of Lactococcus lactis subsp. lactis isolated from surface waters
JF  - Folia Microbiologica
VL  - 53
IS  - 1
SP  - 53-56
EP  - 53-56
PB  - Springer
SN  - 00155632
KW  - Lactococcus lactis subsp. lactis
KW  - water
KW  - taxonomy
KW  - identification
UR  - http://www.biomed.cas.cz/mbu/folia/contents/2008.html
N2  - A group of nine presumptive enterococci was isolated on enterococcal selective media Slanetz-Bartley agar and/or Kanamycin esculin azide agar during a screening of Enterococcus spp. in surface waters. All strains formed a homogeneous cluster separated from all enterococcal species using the rep-PCR fingerprinting with the (GTG)5 primer, but they matched fingerprints revealed by Lactococcus lactis subsp. lactis representatives. Further identification using extensive biotyping and automated ribotyping with EcoRI (RiboPrinter microbial characterization system) confirmed all strains as L. lactis subsp. lactis in full correspondence with the (GTG)5-PCR. We demonstrated that L. lactis subsp. lactis strains occur in different surface waters and can be confused with enterococci due to their positive growth on selective enterococcal media as well as positive results in tests commonly used for identification of the genus Enterococcus (esculin hydrolysis, acetoin and pyrrolidonyl arylamidase production, growth at 10 C and in 6.5 % NaCl). The (GTG)5-PCR fingerprinting revealed as a reliable and fast method for the identification of L. lactis subsp. lactis while automated ribotyping with EcoRI proved to be a good tool for intrasubspecies typing purposes.
ER  -

ŠVEC, Pavel and Ivo SEDLÁČEK. Characterization of Lactococcus lactis subsp. lactis isolated from surface waters. \textit{Folia Microbiologica}. PRINCO International, Praha: Springer, 2008, vol.~53, No~1, p.~53-56. ISSN~0015-5632.

Detailed Information on Publication Record