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@article{764213,
author = {Šebková, Alena and Karasová, Daniela and Crhánová, Magdaléna and Budinská, Eva and Rychlík, Ivan},
article_number = {9},
doi = {http://dx.doi.org/10.1128/JB.00053-08},
keywords = {aro mutant; Salmonella enterica; microarray; gene expression; plasmid},
language = {eng},
issn = {0021-9193},
journal = {JOURNAL OF BACTERIOLOGY},
title = {aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity},
url = {http://jb.asm.org/cgi/content/abstract/190/9/3155},
volume = {190},
year = {2008}
}
TY - JOUR
ID - 764213
AU - Šebková, Alena - Karasová, Daniela - Crhánová, Magdaléna - Budinská, Eva - Rychlík, Ivan
PY - 2008
TI - aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity
JF - JOURNAL OF BACTERIOLOGY
VL - 190
IS - 9
SP - 3155-3160
EP - 3155-3160
PB - American Society for Microbiology
SN - 00219193
KW - aro mutant
KW - Salmonella enterica
KW - microarray
KW - gene expression
KW - plasmid
UR - http://jb.asm.org/cgi/content/abstract/190/9/3155
L2 - http://jb.asm.org/cgi/content/abstract/190/9/3155
N2 - In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA, aroD, and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response.
ER -
ŠEBKOVÁ, Alena, Daniela KARASOVÁ, Magdaléna CRHÁNOVÁ, Eva BUDINSKÁ and Ivan RYCHLÍK. aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity. \textit{JOURNAL OF BACTERIOLOGY}. American Society for Microbiology, 2008, vol.~190, No~9, p.~3155-3160. ISSN~0021-9193. Available from: https://dx.doi.org/10.1128/JB.00053-08.
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