Expresní profily genů c-jun, c-fos a integrinu beta 3 v průběhu diferenciace buněk RAW264.7

SKÁLOVÁ, Kateřina, Petr VAŇHARA and Jan ŠMARDA. Expresní profily genů c-jun, c-fos a integrinu beta 3 v průběhu diferenciace buněk RAW264.7 (Expression profiles of c-jun, c-fos and integrin beta 3 genes in differentiation of RAW264.7 cells.). In Študentská vedecká konferencia, 23.4.2008. Bratislava, Sborník príspevkov. 2008. ISBN 978-80-89238-16-3.

Basic information

Original name

Expresní profily genů c-jun, c-fos a integrinu beta 3 v průběhu diferenciace buněk RAW264.7

Name (in English)

Expression profiles of c-jun, c-fos and integrin beta 3 genes in differentiation of RAW264.7 cells.

Authors

SKÁLOVÁ, Kateřina (203 Czech Republic), Petr VAŇHARA (203 Czech Republic) and Jan ŠMARDA (203 Czech Republic, guarantor)

Edition

Študentská vedecká konferencia, 23.4.2008. Bratislava, Sborník príspevkov. 2008

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Other information

Language

Czech

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/08:00024706

Organization unit

Faculty of Science

ISBN

978-80-89238-16-3

Keywords in English

c-jun; c-fos; integrin beta 3; RAW264.7; differentiation

Tags

c-fos, c-Jun, differentiation, integrin beta 3, RAW264.7

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Abstract

V originále

Naším cílem bylo stanovit dynamiku exprese genů c-jun, c-fos a itgb3 během diferenciace buněk RAW do osteoklastů a dendritických buněk. Genovou expresi jsme studovali na úrovni RNA metodou kvantitativní RT-PCR a na úrovni proteinů westernovým přenosem. Potvrdili jsme, že během diferenciace osteoklastů výrazně stoupá exprese c-fos, zatímco exprese c-jun postupně klesá. V dendritických buňkách je tomu přesně naopak, exprese c-jun stoupá a c-fos klesá. Exprese itgb3 roste v průběhu diferenciace jak osteoklastů, tak dendritických buněk.

In English

The RAW 246.7 murine leukaemic monocyte/macrophage cell line possesses the ability to differentiate into different cell types, such as osteoclasts in RANKL and M-CSF dependent way or dendritic cells (by bacterial lipopolysaccharide). In this process, transcription factors c-Jun and c-Fos are necessary. Integrin beta 3 is important for function of osteoclasts as well by mediating adhesion to the bone surface and the bone matrix resorption. The promotor of integrin beta 3 gene contains binding sites for c-Jun and c-Fos transcription factors as described in in HD11 myeloblasts. Therefore, it is possible that crosstalk between these proteins exists and is important in regulation in osteoclasts activity or differentiation. These molecules can be important in dendritic cell differentiation as well. We studied the expression of c-jun, c-fos a itgB3 during the LPS or M-CSF-RANKL driven differentiation of RAW cells by using quantitative RT-PCR and western-blotting. We will link our results to a functional interactions analysis.

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Links

GA301/06/0036, research and development project	Name: Úloha vybraných transkripčních faktorů v osteoklastové diferenciační dráze Investor: Czech Science Foundation, A role of certain transcription factors in the osteoclastic differentiation pathway
GD204/08/H054, research and development project	Name: Molekulární mechanismy proliferace a diferenciace buněk Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation
MSM0021622415, plan (intention)	Name: Molekulární podstata buněčných a tkáňových regulací Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations