Expresní profily genů c-jun, c-fos a integrinu beta 3 v průběhu diferenciace buněk RAW264.7

SKÁLOVÁ, Kateřina, Petr VAŇHARA and Jan ŠMARDA. Expresní profily genů c-jun, c-fos a integrinu beta 3 v průběhu diferenciace buněk RAW264.7 (Expression profiles of c-jun, c-fos and integrin beta 3 genes in differentiation of RAW264.7 cells.). In Študentská vedecká konferencia, 23.4.2008. Bratislava, Sborník príspevkov. 2008. ISBN 978-80-89238-16-3.

Basic information

• Original name: Expresní profily genů c-jun, c-fos a integrinu beta 3 v průběhu diferenciace buněk RAW264.7
• Name (in English): Expression profiles of c-jun, c-fos and integrin beta 3 genes in differentiation of RAW264.7 cells.
• Authors: SKÁLOVÁ, Kateřina (203 Czech Republic), Petr VAŇHARA (203 Czech Republic) and Jan ŠMARDA (203 Czech Republic, guarantor).
• Edition: Študentská vedecká konferencia, 23.4.2008. Bratislava, Sborník príspevkov. 2008.

Other information

• Original language: Czech
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Slovakia
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/08:00024706
• Organization unit: Faculty of Science
• ISBN: 978-80-89238-16-3
• Keywords in English: c-jun; c-fos; integrin beta 3; RAW264.7; differentiation
• Tags: c-fos, c-Jun, differentiation, integrin beta 3, RAW264.7

Abstract

Naším cílem bylo stanovit dynamiku exprese genů c-jun, c-fos a itgb3 během diferenciace buněk RAW do osteoklastů a dendritických buněk. Genovou expresi jsme studovali na úrovni RNA metodou kvantitativní RT-PCR a na úrovni proteinů westernovým přenosem. Potvrdili jsme, že během diferenciace osteoklastů výrazně stoupá exprese c-fos, zatímco exprese c-jun postupně klesá. V dendritických buňkách je tomu přesně naopak, exprese c-jun stoupá a c-fos klesá. Exprese itgb3 roste v průběhu diferenciace jak osteoklastů, tak dendritických buněk.

Abstract (in English)

The RAW 246.7 murine leukaemic monocyte/macrophage cell line possesses the ability to differentiate into different cell types, such as osteoclasts in RANKL and M-CSF dependent way or dendritic cells (by bacterial lipopolysaccharide). In this process, transcription factors c-Jun and c-Fos are necessary. Integrin beta 3 is important for function of osteoclasts as well by mediating adhesion to the bone surface and the bone matrix resorption. The promotor of integrin beta 3 gene contains binding sites for c-Jun and c-Fos transcription factors as described in in HD11 myeloblasts. Therefore, it is possible that crosstalk between these proteins exists and is important in regulation in osteoclasts activity or differentiation. These molecules can be important in dendritic cell differentiation as well. We studied the expression of c-jun, c-fos a itgB3 during the LPS or M-CSF-RANKL driven differentiation of RAW cells by using quantitative RT-PCR and western-blotting. We will link our results to a functional interactions analysis.

Links

• GA301/06/0036, research and development project: Name: Úloha vybraných transkripčních faktorů v osteoklastové diferenciační dráze

Investor: Czech Science Foundation, A role of certain transcription factors in the osteoclastic differentiation pathway

• GD204/08/H054, research and development project: Name: Molekulární mechanismy proliferace a diferenciace buněk

Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations