VÁŇOVÁ, Lucie and Marie KUMMEROVÁ. Subcellular and organ responses of plants to occurrence of organic pollutant. In Fresh Insights in Plant Affairs - International Conference of PhD Students on Experimental Botany - Book of Abstracts. 2008.
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Basic information
Original name Subcellular and organ responses of plants to occurrence of organic pollutant
Name in Czech Sub-buněčné a orgánové odpovědi rostlin na výskyt organického polutantu
Authors VÁŇOVÁ, Lucie (203 Czech Republic, guarantor, belonging to the institution) and Marie KUMMEROVÁ (203 Czech Republic, belonging to the institution).
Edition Fresh Insights in Plant Affairs - International Conference of PhD Students on Experimental Botany - Book of Abstracts, 2008.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 30105 Physiology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/08:00050969
Organization unit Faculty of Science
Keywords in English fluoranthene; pea plants; chlorophyll fluorescence; hydropony; in vitro
Tags chlorophyll fluorescence, fluoranthene, hydropony, in vitro, pea plants
Changed by Changed by: doc. RNDr. Marie Kummerová, CSc., učo 1146. Changed: 2/4/2012 08:18.
Abstract
The aim was to evaluate the effect of polycyclic aromatic hydrocarbon fluoranthene (FLT, 0.1 and 1 mg/L) on primary processes of photosynthesis in pea plants (Pisum sativum L., cv. Garde) on subcellular (chloroplast suspension) and organ level. Chlorophyll fluorescence measurements were done on suspension of chloroplasts isolated from leaves of plants cultivated hydroponically (Reid-York nutrient solution) and on whole plants cultivated in vitro (Murashige-Skoog medium enriched with 0.1 mg/L IAA or combination of IAA and 0.1 mg/L BA). Both applied concentration of FLT (0.1 and 1 mg/L) added into either chloroplast suspension or MS-medium caused increase in F0 value and decrease in FV/FM and PhiII. The response was more intensive on the chloroplast suspension level compared to imaging of chlorophyll fluorescence distribution on whole plant.
Abstract (in Czech)
Cílem bylo vyhodnotit vliv polycyklického aromatického uhlovodíku fluoranthenu (FLT, 0.1 a 1 mg/L) na primární procesy fotosyntézy u rostlin hrachu (Pisum sativum L., cv. Garde) na subcelulární (suspenze chloroplastů) a orgánové úrovni. Měření fluorescence chlorofylu byla prováděna na suspenzi chloroplastů izolovaných z rostlin kultivovaných hydroponicky (Reid-York živný roztok) a na celých rostlinách kultivovaných in vitro (Murashige-Skoog médium obohacené 0.1 mg/L IAA nebo kombinací IAA a 0.1 mg/L BA). Obě použité koncentrace FLT (0.1 a 1 mg/L), aplikované buď do suspenze chloroplastů nebo do MS-média, vyvolaly zvýšení F0 a pokles FV/FM a PhiII. Citlivější odezva byla zaznamenána na úrovni suspenze chloroplastů v porovnání se zobrazením distribuce fluorescence chlorofylu na celých rostlinách.
Links
MUNI/31/C0007/2007, interní kód MUName: Využití in vitro kultur při studiu vlivu perzistentních organických polutantů na rostliny
Investor: Masaryk University
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